Cover Image

Immobilized affinity ligand techniques /

Bibliographic Details
Main Author: Hermanson, Greg T.
Other Authors: Mallia, A. Krishna, Smith, Paul K. (Paul Keith)
Format: Book
Language:English
Published: San Diego : Academic Press, [1992]
Subjects:
Ligands > Laboratory Manuals.
Chromatography, Affinity > Laboratory Manuals.
Immunosorbent Techniques.
Table of Contents:
  • Matrix
  • General considerations
  • Natural supports
  • Synthetic supports
  • Activation methods
  • General considerations
  • Procedures
  • Immobilization of ligands
  • Small ligands
  • General protein immobilization
  • Immobilization of enzymes
  • Peptide antigens, antibodies, and immunoglobulin binding proteins
  • Immobilization of lectins
  • Immobilized nucleic acids
  • Thiphilic adsorbents
  • Immobilized disulfide reductants
  • Techniques of the trade
  • Measurement of activation level and immobilized ligand density
  • Affinity techniques
  • Selected applications
  • Purification
  • Scavenging
  • Catalysis and modification
  • Analytical applications of affinity ligands.