Flow cytometry and cell sorting /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Berlin ; New York :
Springer-Verlag,
[1992]
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| Series: | Springer laboratory.
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| Subjects: |
Table of Contents:
- Operation of a flow cytometer
- Conjugation of fluorochromes, haptens, and phycobiliproteins to antibodies
- Immunofluorescence : basic considerations
- Multicolor immunofluorescence analysis
- Combined intracellular and surface staining
- Scatchard analysis by flow cytometry
- Preparation and staining of cells for high-resolution DNA analysis
- Simultaneous flow cytometric detection of bromodeoxyuridine incorporation and cell surface marker expression
- High-resolution cell cycle analysis : the Flow Cytometric Bromodeoxyuridine
- Hoechst Quenching Technique
- Cell activation : Indo-1 ratiometric analysis of intracellular ionized calcium
- Biochemical parameters of cell function
- Molecular analysis of transcriptional control : the FACS-Galactosidase assay
- Ligand acidification by nonadherent cells
- Powerful preselection
- High gradient magnetic cell
- Sorting
- Setup of a flow sorter
- Sorting of rare cells
- Fluorescence-activated chromosome sorting
- Flow cytometry and sorting of plant chromosomes
- Large particle sorting
- Biological and laser safety.