PCR : clinical diagnostics and research /
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Berlin ; New York :
Springer-Verlag,
[1992]
|
| Series: | Springer laboratory.
|
| Subjects: |
Table of Contents:
- PCR: principles and reaction components
- Optimization strategies
- General applications of PCR
- Substances affecting PCR: inhibition or enhancement
- PCR: contamination and falsely interpreted results
- Biological material amenable to PCR
- Isolation of DNA from cells and tissue for PCR
- Isolation of RNA from cells and tissue for PCR
- Reverse transcription/PCR (RT-PCR)
- Methods for identification of amplified PCR products
- Restriction fragment analysis
- Multiplex PCR
- Detection of single base changes using PCR
- Non-radioactive, direct, solid-phase sequencing of genomic DNA obtained from PCR
- Application of PCR to analyze unknown sequences
- Quantification of PCR-products
- Cloning methods using PCR
- Site-directed mutagenesis using the PCR
- In-situ polymerase chain reaction
- Oligonucleotides in the field of PCR
- Review of different heat-stable DNA polymerases
- Physical features of thermocyclers and of their influence on the efficiency of PCR amplification
- Alternative methods to PCR.