Protocols in molecular parasitology /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[1993]
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 21. |
| Subjects: |
Table of Contents:
- Culturing and biological cloning of Trypanosoma brucei
- Culturing and biological cloning of Trypanosoma cruzi
- In vitro cultivation and biological cloning of Leishmania
- Simple in vitro cultivation of the malaria parasite Plasmodium falciparum (erythrocytic stages) suitable for large-scale preparations
- Synchronization and cloning of malaria parasites
- Culture and preparation of gametocytes of Plasmodium falciparum for immunochemical, molecular, and mosquito infectivity studies
- Culture of Schistosoma mansoni and production of life cycle stages
- Preparation of DNA and RNA from Trypanosoma brucei
- Isolation of RNA and DNA from Trypanosoma cruzi
- Isolation of DNA and RNA from Leishmania
- Extraction and purification of DNA and RNA from in vitro cultures of the malaria parasite Plasmodium falciparum
- Extraction and purification of nucleic acids from schistosomes
- Application of DNA and whole organisms to filter supports for DNA probe analysis: dot, slot, and touch blotting
- Development and use of repetitive sequences as DNA probes for parasite detection and species identification
- Analysis of the DNA of parasitic protozoa by flow cytometry
- PCR for low-level detection of malaria parasites in blood
- PCR methods for identification of point mutations and gene rearrangements
- Antigenic typing of field isolates of Plasmodium by DNA techniques
- Ribosomal RNA probes for detection and identification of species
- Rapid sequencing of parasite rRNA
- Antibody screening of expression libraries
- Antibody select procedure for characterization of expression clones
- Isolation of parasite genes using synthetic oligonucleotides
- Chromosome mapping methods for parasitic protozoa
- Transfection of Leishmania and Trypanosoma brucei by electroporation
- Two-dimensional polyacrylamide gel electrophoresis
- Isoenzyme electrophoresis for parasite characterization
- Surface labeling of schistosomes
- Monclonal antibody affinity chromotography
- Immunoblotting and enzyme linked immunosorbent assay
- Immunofluorescence of parasites
- Immunoelectron microscopic localization of antigens in malaria parasites
- Preparation of blotted membrane for protein microsequencing
- Identification of T-cell epitopes