rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Protocols in molecular parasitology
  name:
    name part: Hyde, John E.
  type of resource: text
  genre: bibliography
  genre: conference publication
  origin info:
    place:
      place term: nju
    date issued: 1993
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xiv, 470 pages : illustrations ; 23 cm.
  table of contents: Culturing and biological cloning of Trypanosoma brucei -- Culturing and biological cloning of Trypanosoma cruzi -- In vitro cultivation and biological cloning of Leishmania -- Simple in vitro cultivation of the malaria parasite Plasmodium falciparum (erythrocytic stages) suitable for large-scale preparations -- Synchronization and cloning of malaria parasites -- Culture and preparation of gametocytes of Plasmodium falciparum for immunochemical, molecular, and mosquito infectivity studies -- Culture of Schistosoma mansoni and production of life cycle stages -- Preparation of DNA and RNA from Trypanosoma brucei -- Isolation of RNA and DNA from Trypanosoma cruzi -- Isolation of DNA and RNA from Leishmania -- Extraction and purification of DNA and RNA from in vitro cultures of the malaria parasite Plasmodium falciparum -- Extraction and purification of nucleic acids from schistosomes -- Application of DNA and whole organisms to filter supports for DNA probe analysis: dot, slot, and touch blotting -- Development and use of repetitive sequences as DNA probes for parasite detection and species identification -- Analysis of the DNA of parasitic protozoa by flow cytometry -- PCR for low-level detection of malaria parasites in blood -- PCR methods for identification of point mutations and gene rearrangements -- Antigenic typing of field isolates of Plasmodium by DNA techniques -- Ribosomal RNA probes for detection and identification of species -- Rapid sequencing of parasite rRNA -- Antibody screening of expression libraries -- Antibody select procedure for characterization of expression clones -- Isolation of parasite genes using synthetic oligonucleotides -- Chromosome mapping methods for parasitic protozoa -- Transfection of Leishmania and Trypanosoma brucei by electroporation -- Two-dimensional polyacrylamide gel electrophoresis -- Isoenzyme electrophoresis for parasite characterization -- Surface labeling of schistosomes -- Monclonal antibody affinity chromotography -- I…
  note: edited by John E. Hyde.
  note: Includes bibliographical references and index.
  subject:
    topic: Molecular Biology
  subject:
    topic: Parasites
    topic: isolation & purification
  subject:
    topic: Parasitology
    topic: methods
  classification: QL757 .P79 1993
  classification: 574.5/249/0724
  classification: W1 ME9616J v.21 1993 (P) [QX 25 P967 1993]
  classification: QX 25 P967 1993
  related item:
    title info:
      title: Methods in molecular biology (Clifton, N.J.) ; v. 21
  identifier: 0896032396
  identifier: 93008540
  record info:
    record content source: DNLM/DLC
    record creation date: 930511
    record change date: 20191202143707.0
    record identifier: in00004478321