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The baculovirus expression system : a laboratory guide /

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Bibliographic Details
Main Author: King, L. A. (Linda A.), 1959-
Other Authors: Possee, R. D. (Robert D.), 1956-
Format: Book
Language:English
Published: London ; New York : Chapman & Hall, 1992.
Edition:First edition.
Subjects:
Baculoviridae > genetics > Laboratory Manuals.
Gene Expression > Laboratory Manuals.
Table of Contents:
  • The baculoviruses
  • The development of baculovirus expression vectors
  • Processing of foreign proteins synthesized using baculovirus vectors in insect cells
  • Construction of transfer vectors containing the foreign gene
  • Insect cell culture media and maintenance of insect cell lines
  • Propagation, titration and purification of AcMNPV in cell culture
  • Production and selection of recombinant virus
  • Characterization of recombinant viruses
  • Scaling up the production of recombinant protein in insect cells; laboratory bench level
  • Propagation of baculoviruses in insect larvae
  • Trouble-shooting guide.