Western Blotting for the Non-Expert /

This book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfal...

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Bibliographic Details
Main Author: Kurien, Biji T. (Author)
Corporate Author: SpringerLink (Online service)
Format: eBook
Language:English
Published: Cham : Springer International Publishing : Imprint: Springer, 2021.
Edition:1st ed. 2021.
Series:Techniques in Life Science and Biomedicine for the Non-Expert,
Subjects:
Online Access:Connect to the full text of this electronic book
Description
Summary:This book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfalls of western blotting. The book also introduces simple protocols that can transform western blotting into a fun method, such as sending secret messages on membranes or using nitrocellulose membrane as a canvas for art. In addition to the techniques, this book also covers the history of western blotting, which originated from the development of the blotting of DNA. It then delves into the importance of protein blotting, brought to the fore by the fact that the procedure has been evolving constantly since its inception in 1979, and the fact that the scientific community is faced with a multitude of ways and means of transferring proteins to membranes.
Physical Description:1 online resource (XXIII, 467 pages 611 illustrations, 170 illustrations in color.)
ISBN:9783030706845
ISSN:2367-1122
DOI:10.1007/978-3-030-70684-5