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In situ analysis of cellular functional molecules /

This book introduces the tailor-made design of detection probes as well as schemes from a top-down perspective according to the unique characteristics of cellular functional molecules.

Bibliographic Details
Main Authors: Ju, Huangxian (Author), Ding, Lin (Author), Tang, Bo (Author)
Format: eBook
Language:English
Published: Cambridge : Royal Society of Chemistry, [2020]
Series:RSC detection science series ; no. 16.
Subjects:
Cytology > Analysis.
Molecular biology.
Molecules.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Intro
  • Title
  • Copyright
  • Preface
  • Contents
  • Chapter 1 Design of In Situ Cytosensing Strategies
  • 1.1 General Principles of In Situ Cytosensing
  • 1.2 Enhancement of Detection Specificity
  • 1.3 Design of Off-On Signal Switch
  • 1.4 Design of Signal Amplification Strategy
  • 1.5 Design of Multi-channel Analysis
  • 1.6 Perspective
  • References
  • Chapter 2 In Situ Detection of Cell-surface Glycans
  • 2.1 Introduction to Cell-surface Glycans
  • 2.1.1 Structures and Classification of Glycans
  • 2.1.2 Functions of Glycans
  • 2.2 Labelling and Recognition of Glycans
  • 2.2.1 Lectin Recognition
  • 2.2.2 Chemoselective Labelling
  • 2.2.3 Metabolic Oligosaccharide Engineering
  • 2.2.4 Chemoenzymatic Glycan Labelling
  • 2.3 Global Analysis of Cell-surface Glycans
  • 2.4 Competition Systems for Quantification of Cell-surface Glycans
  • 2.5 Protein-specific Imaging of Cell-surface glycans
  • 2.6 Perspective
  • References
  • Chapter 3 In Situ Detection of Intracellular Messenger RNA and MicroRNA
  • 3.1 Introduction
  • 3.2 Biofunction of mRNA and miRNA
  • 3.2.1 Biofunction of mRNA
  • 3.2.2 Biofunction of miRNA
  • 3.3 Viral Gene Vectors
  • 3.3.1 Lenti-/Retrovirus-mediated Vectors for Gene Delivery
  • 3.3.2 Adenovirus-mediated Vectors for Gene Delivery
  • 3.3.3 Adeno-associated Virus Vectors for Gene Delivery
  • 3.4 Organic Gene Vectors
  • 3.4.1 Cationic Polymers
  • 3.4.2 Cationic Lipids
  • 3.5 Inorganic Gene Vectors
  • 3.5.1 Gold-based Nanomaterials as Gene Vectors
  • 3.5.2 Two-dimensional GO and TMD Nanosheets as Gene Vectors
  • 3.6 Intracellular mRNA Detection
  • 3.7 Intracellular miRNA Detection
  • 3.7.1 "One-to-One" Signal Detection
  • 3.7.2 Sensitive Detection Based on Signal Amplification
  • 3.8 Perspective and Challenges
  • 4.7 Perspective
  • Acknowledgements
  • References
  • Chapter 5 In Situ Analysis of the Intracellular Caspase Family
  • 5.1 Introduction to the Intracellular Caspase Family
  • 5.2 Imaging of Caspase Activity in Living Cells
  • 5.2.1 FRET-based Sensors for Imaging of Intracellular Caspase Activity
  • 5.2.2 Fluorescence Lifetime Imaging Microscopy for Imaging of Intracellular Caspase Activity
  • 5.2.3 AIE Probe for Imaging of Intracellular Caspase Activity
  • 5.2.4 Two-photon (TP) Imaging of Intracellular Caspase Activity
  • Acknowledgements
  • References
  • Chapter 4 In Situ Analysis of Intracellular Telomerase Activity
  • 4.1 Clinical Significance of Telomerase
  • 4.2 Traditional Analytical Methods for Telomerase
  • 4.3 In Situ Imaging of Intracellular Telomerase Activity Based on Functional Nanoprobes
  • 4.4 In Situ Imaging of Intracellular Telomerase Activity Through FRET
  • 4.4.1 FRET Based on Organic Quenchers
  • 4.4.2 FRET Based on AuNPs
  • 4.5 Raman Spectroscopy-based Intracellular Telomerase Activity Sensing
  • 4.6 In Situ Monitoring of Intracellular Telomerase Activity Using AIEgen-based Bioprobes