http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
In situ analysis of cellular functional molecules
This book introduces the tailor-made design of detection probes as well as schemes from a top-down perspective according to the unique characteristics of cellular functional molecules.
Intro -- Title -- Copyright -- Preface -- Contents -- Chapter 1 Design of In Situ Cytosensing Strategies -- 1.1 General Principles of In Situ Cytosensing -- 1.2 Enhancement of Detection Specificity -- 1.3 Design of Off-On Signal Switch -- 1.4 Design of Signal Amplification Strategy -- 1.5 Design of Multi-channel Analysis -- 1.6 Perspective -- References -- Chapter 2 In Situ Detection of Cell-surface Glycans -- 2.1 Introduction to Cell-surface Glycans -- 2.1.1 Structures and Classification of Glycans -- 2.1.2 Functions of Glycans -- 2.2 Labelling and Recognition of Glycans
2.2.1 Lectin Recognition -- 2.2.2 Chemoselective Labelling -- 2.2.3 Metabolic Oligosaccharide Engineering -- 2.2.4 Chemoenzymatic Glycan Labelling -- 2.3 Global Analysis of Cell-surface Glycans -- 2.4 Competition Systems for Quantification of Cell-surface Glycans -- 2.5 Protein-specific Imaging of Cell-surface glycans -- 2.6 Perspective -- References -- Chapter 3 In Situ Detection of Intracellular Messenger RNA and MicroRNA -- 3.1 Introduction -- 3.2 Biofunction of mRNA and miRNA -- 3.2.1 Biofunction of mRNA -- 3.2.2 Biofunction of miRNA -- 3.3 Viral Gene Vectors
3.3.1 Lenti-/Retrovirus-mediated Vectors for Gene Delivery -- 3.3.2 Adenovirus-mediated Vectors for Gene Delivery -- 3.3.3 Adeno-associated Virus Vectors for Gene Delivery -- 3.4 Organic Gene Vectors -- 3.4.1 Cationic Polymers -- 3.4.2 Cationic Lipids -- 3.5 Inorganic Gene Vectors -- 3.5.1 Gold-based Nanomaterials as Gene Vectors -- 3.5.2 Two-dimensional GO and TMD Nanosheets as Gene Vectors -- 3.6 Intracellular mRNA Detection -- 3.7 Intracellular miRNA Detection -- 3.7.1 "One-to-One" Signal Detection -- 3.7.2 Sensitive Detection Based on Signal Amplification -- 3.8 Perspective and Challenges
4.7 Perspective -- Acknowledgements -- References -- Chapter 5 In Situ Analysis of the Intracellular Caspase Family -- 5.1 Introduction to the Intracellular Caspase Family -- 5.2 Imaging of Caspase Activity in Living Cells -- 5.2.1 FRET-based Sensors for Imaging of Intracellular Caspase Activity -- 5.2.2 Fluorescence Lifetime Imaging Microscopy for Imaging of Intracellular Caspase Activity -- 5.2.3 AIE Probe for Imaging of Intracellular Caspase Activity -- 5.2.4 Two-photon (TP) Imaging of Intracellular Caspase Activity
Acknowledgements -- References -- Chapter 4 In Situ Analysis of Intracellular Telomerase Activity -- 4.1 Clinical Significance of Telomerase -- 4.2 Traditional Analytical Methods for Telomerase -- 4.3 In Situ Imaging of Intracellular Telomerase Activity Based on Functional Nanoprobes -- 4.4 In Situ Imaging of Intracellular Telomerase Activity Through FRET -- 4.4.1 FRET Based on Organic Quenchers -- 4.4.2 FRET Based on AuNPs -- 4.5 Raman Spectroscopy-based Intracellular Telomerase Activity Sensing -- 4.6 In Situ Monitoring of Intracellular Telomerase Activity Using AIEgen-based Bioprobes
Includes bibliographical references and index.