Cover Image

Lipidomics : current and emerging techniques /

Lipidomics is one of the emerging 'omics' techniques. This book will provide a guide to this growing area and its importance to the biosciences.

Bibliographic Details
Other Authors: Griffiths, William (Editor), Wang, Yuqin (Editor)
Format: eBook
Language:English
Published: Cambridge : Royal Society of Chemistry, [2020]
Series:New developments in mass spectrometry.
Subjects:
Lipids > Analysis > Technique.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Intro
  • Half Title
  • Series editor
  • Title
  • Copyright
  • Contents
  • Chapter 1 Lipidomics Basics
  • 1.1 Introduction
  • 1.2 Most Common Experimental Methods in Lipidomics
  • 1.2.1 DI and Shotgun Methods
  • 1.2.2 LC-MS Methods
  • 1.2.3 GC-MS Methods
  • 1.3 Emerging Experimental Methods in Lipidomics
  • 1.3.1 Low-flow-rate-LC
  • 1.3.2 IM-MS
  • 1.3.3 MSI
  • 1.4 Sample Preparation and Derivatisation
  • 1.4.1 Liquid and Solid Phase Extractions
  • 1.4.2 Liquid Extraction for Surface Analysis (LESA)
  • 1.4.3 LESA for MSI
  • 1.4.4 Derivatisation for Lipidomics
  • 1.5 Databases Searches, Nomenclature and Reporting
  • 1.6 Conclusions
  • Acknowledgements
  • References
  • Chapter 2 Multivariate Statistics in Lipidomics
  • 2.1 Introduction
  • 2.2 The Need for Multivariate Statistics: Why Doesn't a Students t-test Do Everything?
  • 2.3 Commonly Used Multivariate Statistical and Pattern Recognition Techniques
  • 2.3.1 Unsupervised and Supervised Forms of Multivariate Statistics
  • 2.3.2 Principal Components Analysis
  • 2.3.3 Hierarchical Cluster Analysis
  • 2.3.4 Partial Least Squares Discriminant Analysis and Its Extensions
  • 2.3.5 Random Forest and Machine Learning Approaches
  • 2.4 Application of Multivariate Statistics to Lipidomics
  • 2.4.1 Direct Infusion Mass Spectrometry
  • 2.4.2 Liquid Chromatography Mass Spectrometry
  • 2.4.3 Ion Mobility
  • 2.4.4 Pattern Recognition in Mass Spectrometry Imaging
  • 2.5 Workflow Tools for Lipidomics
  • 2.6 Conclusions
  • References
  • Chapter 3 Low-flow Rate Separations of Lipids
  • 3.1 Introduction
  • 3.2 Low-flow Liquid Chromatography and Lipids: Microbore, Capillary, Nano, Open Tubular Column Formats
  • 3.2.1 Low-flow Liquid Chromatography: A Tool for High Sensitivity and Small Sample Analysis
  • 3.2.2 Capillary Liquid Chromatography: Lipid Applications
  • 3.2.3 Temperature as a Tool in Capillary Liquid Chromatography for Lipid Analysis
  • 3.2.4 Coupling Capillary Liquid Chromatography and Electrospray Mass Spectrometry for Lipidomics
  • 3.2.5 Expanding Low-flow Chromatography: Automated Sample Preparation and Multi-dimensional Separations of Lipids
  • 3.3 Capillary Zone Electrophoresis, Micellar Electrokinetic Chromatography, Capillary Electrochromatography, for Lipid Separations
  • 3.3.1 Capillary Zone Electrophoresis
  • 3.3.2 Micellar Electrokinetic Capillary Chromatography and Related Approaches
  • 3.3.3 Capillary Electrochromatography and Lipids
  • 3.4 The Future of Lipids and Low-flow Separations
  • References
  • Chapter 4 Ion Mobility-Mass Spectrometry for Lipid Analysis
  • 4.1 Introduction
  • 4.2 IM-derived CCS
  • 4.3 IM as an Additional Dimension of Separation
  • 4.3.1 Online Separation-IM-MS
  • 4.3.2 IM-MS in Shotgun Lipidomics
  • 4.3.3 Desorption Ionization with IM-MS
  • 4.4 IM in Combination with Tandem Mass Spectrometry
  • 4.4.1 Post-mobility Fragmentation