New and future developments in microbial biotechnology and bioengineering : penicillium system properties and applications /
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
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Amsterdam, Netherlands ; Cambridge, MA, United States :
Elsevier,
[2018]
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| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Front Cover; New and Future Developments in Microbial Biotechnology and Bioengineering; Copyright Page; Contents; List of Contributors; I. Penicillium: Biology to Biotechnology; 1 Biodiversity of the Genus Penicillium in Different Habitats; 1.1 Introduction; 1.2 Isolation and Characterization of Penicillium; 1.3 Molecular Diversity and Phylogenetic Analysis; 1.4 Distribution and Abundance of Penicillium; 1.4.1 Low/High-Temperature Habitat; 1.4.2 Saline Habitat; 1.4.3 Acidic/Alkaline Habitat; 1.4.4 Penicillium Associated With Plants and Fruits; 1.5 Conclusion and Future Prospects; References
- 2 Understanding the Diversity of Penicillium Using Next-Generation Sequencing 2.1 Introduction; 2.2 Isolation of Penicillium species; 2.2.1 Isolation From Air; 2.2.2 Isolation From Soil Samples; 2.2.3 Isolation From Food Samples; 2.3 Detection of Penicillium species; 2.3.1 Conventional Techniques; 2.3.2 Molecular Techniques; 2.3.2.1 Preparation of Fungal Culture for Toxin Detection; 2.3.2.2 DNA Extraction From Fungal Cultures for Molecular Identification; 2.3.2.3 Determining DNA Concentration and Purity; 2.3.2.4 Polymerase Chain Reaction; 2.3.2.5 PCR Detection of Penicillium Species
- 2.4.3.1.3 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Fingerprinting (MALDI-TOF MS)2.4.3.2 Cloning and Sequencing of PCR Products; 2.4.3.2.1 Preparation and Analysis of DNA Heteroduplexes; 2.4.3.2.2 Identification of Polymorphic Genetic Loci; 2.4.3.3 Next-Generation Sequencing; 2.4.3.3.1 Roche 454 FLX Pyrosequencer; 2.4.3.3.2 Illumina Genome Analyzer; 2.4.3.3.3 Applied Biosystem SOLiD Sequencer; 2.4.3.3.4 Heliscope Single-Molecule Sequencer; 2.4.3.3.5 Ion Personal Genome Machine (PGM); 2.4.3.3.6 RNA-Seq
- 2.4.3.3.7 Pacific Biosciences SMRT Sequencing DNA Sequencer2.4.3.4 Submitting DNA Fragment for Sequencing; 2.4.3.4.1 Using Bioinformatics; 2.5 Conclusions and Future Perspectives; References; Further Reading; II. Secondary Metabolism; 3 Secondary Metabolism and Antimicrobial Metabolites of Penicillium; 3.1 Introduction; 3.2 Secondary Metabolism in Fungi; 3.2.1 Secondary Metabolism as Differentiation; 3.3 Microorganisms as Sources of Secondary Metabolites; 3.3.1 Bioactivities of Microbial Secondary Metabolites; 3.3.2 Biotechnological Importance of Fungi; 3.3.2.1 Ergot Alkaloids