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Field guide to microscopy /

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This guide provides extensive coverage of microscopic imaging principles. After reviewing the main principles of image formation, diffraction, interference, and polarization used in microscopy, this guide describes the most widely applied microscope configurations and applications. It also covers ma...

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Bibliographic Details
Main Author: Tkaczyk, Tomasz S.
Corporate Author: Society of Photo-optical Instrumentation Engineers
Format: eBook
Language:English
Published: Bellingham, Wash. (1000 20th St. Bellingham WA 98225-6705 USA) : SPIE, [2010]
Series:SPIE field guides ; FG13.
Subjects:
Microscopy > Handbooks, manuals, etc.
Electronic books.
Online Access:Connect to the full text of this electronic book
Description
Summary:This guide provides extensive coverage of microscopic imaging principles. After reviewing the main principles of image formation, diffraction, interference, and polarization used in microscopy, this guide describes the most widely applied microscope configurations and applications. It also covers major system components, including light sources, illumination layouts, microscope optics, and image detection electronics. This guide also provides a comprehensive overview of microscopy techniques, including bright field and dark field imaging, contrast enhancement methods (such as phase and amplitude contrast), DIC, polarization, and fluorescence microscopy. In addition, it describes scanning techniques (such as confocal and multiphoton imaging points); new trends in super-resolution methods (such as 4Pi microscopy, STED, STORM, and structured illumination); and array microscopy, CARS, and SPIM.
Item Description:"SPIE digital library."
Electronic resource.
Physical Description:1 online resource (xv, 138 pages) : illustrations.
Also available in print version.
Format:Mode of access: World Wide Web.
System requirements: Adobe Acrobat Reader.
Bibliography:Includes bibliographical references (pages 128-132) and index.
ISBN:9780819478917 (electronic)
DOI:10.1117/3.798239
Access:Restricted to subscribers or individual electronic text purchasers.