Heterologous Expression of Membrane Proteins : Methods and Protocols /
This detailed volume encompasses chapters from leading experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional production and stabilization of recombinant integral membrane proteins (IMPs). Membrane proteins play a key rol...
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| Format: | eBook |
| Language: | English |
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New York, NY :
Springer New York : Imprint: Humana Press,
2016.
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| Edition: | 2nd ed. 2016. |
| Series: | Methods in molecular biology.
1432. |
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| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies
- Membrane Protein Production in the Yeast, S. cerevisiae
- Membrane Proteins Production in Escherichia coli: Protocols and Rules
- Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach
- Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins
- Membrane Protein Production in Lactococcus lactis for Functional Studies
- Expression of Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells
- Leishmania tarentolæ as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6
- Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA
- Expression of Eukaryotic Membrane Proteins in Pichia pastoris
- Integral Membrane Protein Expression in Saccharomyces cerevisiae
- High-Throughput Baculovirus Expression System for Membrane Protein Production
- Small-Scale Screening to Large-Scale Over-Expression of Human Membrane Proteins for Structural Studies
- Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies
- Membrane Protein Solubilization and Composition of Protein Detergent Complexes
- Detergent-Free Membrane Protein Purification
- Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry
- Lessons from an a-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization
- Method to Screen Multidrug Transport Inhibitors Using Yeast Over-Expressing a Human MDR Transporter.