Auditory and Vestibular Research : Methods and Protocols /

This second edition expands upon the previous volume with new and updated chapters. Auditory and Vestibular Research: Methods and Protocols, Second Edition guides readers through protocols on cell culture, tissue engineering, nanotechnology, high-throughput screening, and physiology. Chapters on phy...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Sokolowski, Bernd (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana Press, 2016.
Edition:2nd ed. 2016.
Series:Methods in molecular biology. 1427.
Subjects:
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • HeliosĀ® Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: Recent Updates
  • Tol2-Mediated Delivery of miRNAs to the Chicken Otocyst using Plasmid Electroporation
  • A Rapid, Cost-Effective Method of Recombinant Adeno-Associated Virus Preparation Enables Efficient Gene Transfer to the Developing Mouse Inner Ear
  • Generation of Noninvasive, Quantifiable, Orthotopic Animal Models for NF2-Associated Schwannoma and Meningioma
  • RNA Extraction from Xenopus Auditory and Vestibular Organs for Molecular Cloning and Expression Profiling with RNA-Seq and Microarrays
  • Yeast Two-Hybrid Screening to Test for Protein-Protein Interactions in the Auditory System
  • Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research
  • Protein Quantification of the Developing Cochlea by Mass Spectrometry
  • Ultrastructural Identification and Colocalization of Interacting Proteins in the Murine Cochlea by Post-Embedding Immunogold Transmission Electron Microscopy
  • Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Inner-Ear Proteins
  • The Single-Molecule Approach to Membrane Protein Stoichiometry
  • Visualization of Live Cochlear Stereocilia at a Nanoscale Resolution Using Hopping Probe Ion Conductance Microscopy
  • Design and Construction of a Cost-Effective Spinning Disk System for Live Imaging of Inner Ear Tissue
  • Neuroanatomical Tracing Techniques: History, State of the Art, and Future Developments
  • Detection of Excitation and Inhibition in the Auditory System Using Fluorescence Immunohistochemistry and High Resolution Fluorescence Microscopy
  • Rapid and Semi-Automated Extraction of Neuronal Cell Bodies and Nuclei from Electron Microscopy Image Stacks.-Organotypic Culture of the Mouse Cochlea from Embryonic Day 12 to the Neonate
  • Quantifying Spiral Ganglion Neurite and Schwann Behavior on Micropatterned Polymer Substrates
  • The Use of Human Wharton's Jelly Cells for Cochlear Tissue Engineering
  • Nanotechnology in Auditory Research: Membrane Electromechanics in Hearing
  • Overview of Nanoparticle Based Delivery for Treatment of Inner Ear Disorders
  • Development of Cell-based High-Throughput Chemical Screens for Protection Against Cisplatin-induced Ototoxicity.-Profiling Specific Inner Ear Cell Types Using Cell Sorting Techniques
  • Optical Coherence Tomography to Measure Sound-Induced Motions Within the Mouse Organ of Corti In Vivo
  • Method for Dissecting the Auditory Epithelium (Basilar Papilla) in Developing Chick Embryos
  • Whole Cell Patch Clamp Recording of Mouse and Rat Inner Hair Cells in the Intact Organ of Corti
  • Glass Probe Stimulation of Hair Cell Stereocilia
  • A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells
  • In Vitro Functional Assessment of Adult Spiral Ganglion Neurons (SGNs)
  • Mapping Auditory Circuits with Photostimulation of Caged Glutamate. .