rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Advances and Clinical Practice in Pyrosequencing
  name:
    name part: Song, Qinxin
    role:
      role term: editor.
  name:
    name part: Zhou, Guohua
    role:
      role term: editor.
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  genre: Electronic books.
  origin info:
    place:
      place term: xxu
    date issued: 2016
    edition: 1st ed. 2016.
    issuance: monographic
  language:
    language term: eng
  physical description:
    reformatting quality: access
    form: remote
    extent: 1 online resource (XIV, 407 pages 156 illustrations, 44 illustrations in color.)
  abstract: The aim of this book is to improve pyrosequencing protocols as well as instrumentation for better clinical use by describing improvements and novel applications of pyrosequencing technology. Divided into five parts, the book's thirty chapters explore advances in pyrosequencing template preparation, pyrosequencing technology innovations, multiplex pyrosequencing based on barcodes, the miniaturization of pyrosequencing equipment, as well as various applications. As part of the Springer Protocols program, chapters contain the kind of detail and practical implementation advice to guarantee successful results in the lab. Comprehensive and thorough, Advances and Clinical Practice in Pyrosequencing serves as a valuable reference for researchers who are engaged in personalized medicine, disease control, and DNA diagnosis in numerous other fields.
  table of contents: Preparation of single-stranded DNA for pyrosequencing by LATE-PCR -- A simplified protocol for preparing pyrosequencing templates based on LATE-PCR using whole blood as starting material directly -- Improvement of LATE-PCR to prepare pyrosequencing template -- Pyrosequencing-templates generated by nicking PCR products with nicking endonuclease -- Pyrosequencing-templates generated by asymmetric Nucleic Acid Sequence-Based Amplification (asymmetric-NASBA) -- Gel immobilization of acrylamide-modified single-stranded DNA template for pyrosequencing -- Multiplex PCR based on a universal biotinylated primer to generate templates for pyrosequencing -- A novel pyrosequencing principle based on AMP-PPDK reaction for improving the detection limit -- Pyrosequencing chemistry coupled with modified primer extension reactions for quantitative detection of allele frequency in a pooled sample -- A gel-free SNP genotyping method based on pyrosequencing chemistry coupled with modified primer extension reactions directly from double-stranded PCR products -- Improvement of pyrosequencing to allow multiplex SNP typing in a program -- Improvement of pyrosequencing-sensitivity based on the capture of free adenosine 5'-phosphosulfate with adenosine triphosphate sulfurylase -- Using polymerase preference index to design imLATE-PCR primers for an efficient pyrosequencing -- Preparation of thermalstable biotinlyted firefly luciferase and its application in pyrosequencing -- Expression of thermostable recombinant luciola lateralis luciferase and its function on setting up of heat-stable pyrosequencing system -- Expression and purification of ATP sulfurylase from saccharomyces cerevisias in Escherichia coli and its application in pyrosequencing -- Characterization of recombinant Escherichia coli single-strand binding protein and its application in pyrosequencing -- Expression of PPDK from Microbispora Rosea Subsp.aerata in E.coli and its application in pyrosequencing -- Construction of 3-plex …
  note: edited by Guohua Zhou, Qinxin Song.
  note: Electronic resource.
  subject:
    topic: Biomedicine
  subject:
    topic: Human Genetics
  subject:
    topic: Human genetics
  subject:
    topic: Medicine
  classification: QP625.N89 A383 2016
  classification: 599.935
  classification: 611.01816
  related item:
    title info:
      title: Springer eBooks
  related item:
    title info:
      title: Springer protocols (Series)
  identifier: 9781493933082
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record creation date: 160204
    record change date: 20210728130327.0
    record identifier: in00003713274