Baculovirus and Insect Cell Expression Protocols /
The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum...
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| Format: | eBook |
| Language: | English |
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New York, NY :
Springer New York : Imprint: Humana Press,
2016.
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| Edition: | 3rd ed. 2016. |
| Series: | Methods in molecular biology.
1350. |
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| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Useful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior
- Introduction to Baculovirus Molecular Biology
- Baculovirus Transfer Vectors
- Recombinant Baculovirus Isolation
- Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System
- Available Lepidopteran Insect Cell Lines
- Lepidopteran Insect Cell Line Isolation from Insect Tissue
- Development Of Serum-Free Media For Lepidopteran Insect Cell Lines
- Routine Maintenance And Storage Of Lepidopteran Insect Cell
- Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System
- Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System
- Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors
- Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae
- Production Of Virus-Like Particles For Vaccination
- Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells
- Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression
- Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression
- Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression
- Introduction To The Use Of Baculoviruses As Biological Insecticides
- Baculovirus Insecticide Production In Insect Larvae
- Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses
- Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants
- Tubular Bioreactor for Probing Baculovirus Infection and Protein Production
- Gene Silencing In Insect Cells Using Rnai
- Using the Baculovirus/Insect Cell System to Study Apoptosis
- Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells.