Cover Image

A journey through genetics. Part II /

Bibliographic Details
Main Author: Moitra, Karobi (Author)
Format: eBook
Language:English
Published: San Rafael, California (1537 Fourth Street, San Rafael, CA 94901 USA) : Morgan & Claypool, 2014.
Series:Colloquium digital library of life sciences.
Colloquium series on the genetic basis of human disease ; # 6.
Subjects:
DNA > History.
Genetics > History.
Human genome.
DNA > history.
Genetics > history.
Genome, Human.
DNA
chromatin
gene cloning
genetic diversity
genetics
human genome project
nucleosome
operon
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • History of genetics timeline (part 2)
  • 1. The age of the operon: the regulation of gene expression in E. coli
  • 1.1 The operon model: the lactose operon of E. coli
  • 1.2 Structural elements of the Lac operon
  • 1.2.1 Structural genes (Lac Z, Lac Y, Lac A)
  • 1.2.2 The operator gene (Lac O)
  • 1.2.3 The regulator gene (Lac I)
  • 1.3 Functional regulation of the lactose operon
  • 1.3.1 The role of induction and repression in the Lac operon
  • 1.3.2 Attachment of the RNA polymerase
  • 1.3.3 Transcription of the structural genes
  • 1.4 A short educational skit based on the lactose operon: I need energy: a tale of two sugars
  • 1.5 What would happen if both glucose and lactose were present in the medium?
  • 2. Genetic diversity: cheetah chatter
  • 2.1 The journey into Africa
  • 2.2 Proteins in the cheetah's blood were separated and analyzed using a technique called protein gel electrophoresis
  • 2.3 A second line of evidence from MHC proteins and skin grafts
  • 2.4 Genetic diversity
  • 2.5 Cheetahs and the bottleneck effect
  • Gene cloning and recombinant DNA technology
  • 3.1 Gene manipulation/genetic engineering can be divided into two steps (figure 3.1)
  • 3.2 Characteristic features of cloning vectors
  • 3.3 Applications of genetic engineering
  • 4. Applications of genetic engineering, resurrecting the influenza virus
  • 4.1 The journey
  • 4.1.1 Resurrection
  • 4.1.2 The one that got away
  • 4.1.3 Reverse engineering a killer virus
  • 5. The human journey
  • 5.1 Spencer Wells and the genographic project
  • 5.1.1 In the beginning
  • 5.2 How do we know that this amazing journey started in Africa?
  • 5.2.1 Mapping markers on Y chromosomes
  • 5.2.2 STR markers
  • 5.2.3 Mitochondrial Eve and female mitochondrial markers
  • 5.2.4 Mapping single nucleotide polymorphisms on mitochondrial DNA
  • 5.2.5 The journey of the y chromosome (figure 5.5)
  • 5.2.6 The journey out of Africa (the M168 marker)
  • 5.2.7 The first route out of Africa and on to Australia
  • 5.2.8 Journeying on to Australia (M130 marker)
  • 5.2.9 The second route out of Africa to Central Asia
  • 5.2.10 Forty thousand years ago modern humans reach Central Asia
  • 5.2.11 Following the M45 (migrating East)
  • 5.2.12 The longest journey: 50,000 years and people reach South America (the M3 marker)
  • 5.2.13 What did you learn from this journey?
  • 6. The three-dimensional folding of the genome
  • 6.1 Beads on a string
  • 6.1.1 The nucleosome concept: the first level of folding
  • 6.1.2 Nucleosomes unraveled
  • 6.2 Linker histone and linker DNA
  • 6.3 Higher-order folding of chromatin
  • 6.3.1 The 30-nm fiber of chromatin
  • 6.3.2 Solenoid model
  • 6.3.3 Conventional model
  • 6.4 Erez Lieberman Aiden and the three-dimensional folding of the genome
  • 6.5 Hi-C in detail: a method to study the three-dimensional architecture of genomes
  • 6.5.1 Hi-C method
  • 6.6 "The genome is Scottish!" Eric Lander
  • 6.6.1 Why is the genome Scottish? Of Ramen noodles and tangled ear buds
  • 6.6.2 The old theory, the equilibrium globule debunked
  • 7. The human genome project and beyond
  • 7.1 Goals of the human genome project
  • 7.2 Cost of the human genome project
  • 7.2.1 The race to the human genome
  • 7.3 How was the human genome sequenced?
  • 7.4 Sequencing strategies
  • 7.4.1 Hierarchical shotgun sequencing strategy
  • 7.4.2 Whole-genome shot gun sequencing
  • 7.5 The human genome project: what did we learn?
  • 7.6 Early DNA sequencing technologies
  • 7.6.1 Sanger sequencing
  • 7.6.2 Dye terminator cycle sequencing
  • 7.6.3 Beyond the human genome project: next-generation sequencing strategies
  • 7.6.4 Next-generation sequencing (NGS) technologies
  • 7.7 Applications of NextGen sequencing
  • References
  • Author biography.