Table of Contents:
  • 1. Compartmentalization is a hallmark of eukaryotic cells
  • 2. Organelles can be classified based on their origin
  • 3. There are multiple entry points into the endomembrane system and a network of connecting trafficking routes
  • 3.1 The exocytic pathway
  • 3.2 The endocytic pathway
  • 3.3 The ER-peroxisome pathway
  • 4. There are exceptions and extensions to the classic trafficking itineraries
  • 4.1 Unconventional secretion
  • 4.2 Vesicular transport from mitochondria to peroxisomes and lysosomes
  • 5. A mathematical model can explain how organelle size is established and maintained
  • 6. Coats and snares constitute the core machinery for vesicle budding and fusion
  • 6.1 Vesicle formation
  • 6.2 Vesicle fission
  • 6.3 Vesicle fusion
  • 7. A mathematical model explains how coats and snares are sufficient to generate organelle identity
  • 8. How vesicle formation is linked to cargo incorporation
  • 8.1 How to avoid empty vesicles
  • 8.2 The nature of coat-cargo interactions
  • 8.3 Clathrin coated vesicles, endocytosis and exit from the TGN
  • 8.4 COPII vesicles, exit from the ER
  • 8.5 COPI vesicles, ER retrieval and intra-Golgi transport
  • 8.6 Sorting roles of transmembrane domains
  • 8.7 Sugars as luminal sorting determinants
  • 9. RabGTPases are the master regulators of vesicular traffic
  • 9.1 Rab-mediated regulation of coat-cargo recruitment
  • 9.2 Vesicle uncoating and tethering
  • 9.3 Rab proteins and the actin and microtubule (MT) cytoskeleton in vesicular traffic
  • 9.4 Rab proteins and phosphoinositide lipids
  • 9.5 Rab domains and Rab cascades
  • 10. The mechanisms and physiological roles of endocytosis
  • 11. Models for protein transport in the Golgi apparatus
  • 12. Summary
  • Acknowledgments
  • References
  • Author biography.