Membrane nanodomains /

Bibliographic Details
Main Author:	Silvius, John Robert, 1954-
Format:	eBook
Language:	English
Published:	San Rafael, Calif. (1537 Fourth Street, San Rafael, CA 94901 USA) : Morgan and Claypool, [2013]
Series:	Colloquium digital library of life sciences. Colloquium series on building blocks of the cell ; # 1.
Subjects:	Cell membranes. Cell Membrane. biological membrane membrane lipids membrane proteins membrane curvature lipid phases lipid rafts cholesterol protein-protein interactions protein-lipid interactions fluorescence microscopy electron microscopy single-particle fluorescence super-resolution microscopy mass spectrometry epithelial cells lymphocytes mast cells cell signaling membrane traffic Electronic books.
Online Access:	Connect to the full text of this electronic book

Table of Contents:

1. Introduction
1.1 Notes on terminology
2. Thermodynamic bases for formation of membrane domains
2.1 Lipid segregation in model membranes
2.2 Lipid-protein interactions
2.2.1 Interactions of intramembrane protein domains with lipids
2.2.2 Interactions of extramembranous proteins and protein domains with lipids
2.2.3 Determinants of protein association with liquid-ordered vs liquid-disordered lipids
2.2.4 Association of proteins and lipids with highly curved membrane structures
2.3 Protein-protein interactions in membranes
2.4 Membrane 'scaffold'-forming proteins
3. Kinetic bases for formation of membrane domains
4. Historical development of the concept of membrane nanodomains
5. Experimental tools to study nanodomain formation in cells
5.1 Biochemical fractionation methods
5.2 Fluorescence microscopy and colocalization analysis
5.3 Manipulations of membrane lipid composition
5.4 Lipid model systems
5.5 Electron-microscopic methods and analysis of molecular clustering
5.6 Fluorescence resonance energy transfer
5.7 Bimolecular fragment complementation (BIFC) microscopy
5.8 Single-particle measurements of molecular diffusion and interactions
5.9 Super-resolution light microscopies
5.10 Plasma membrane-derived giant vesicles
5.11 Lipid mass spectrometry (MS)
6. Experimental studies of nanodomains in cellular systems
6.1 Membrane domain organization in fibroblasts
6.2 Nanocluster-based signaling by GPI - and Ras proteins
6.3 Endocytic trafficking of 'raft' components in fibroblasts
6.4 Polarized trafficking of apically localized proteins in renal epithelial cells
6.5 Activation of the T-cell receptor
6.6 Mast cells and the FceRI receptor
7. Looking forward
References.