Cover Image

Biobanking in the era of the stem cell : a technical and operational guide /

Bibliographic Details
Main Author: Moore, Jennifer C.
Other Authors: Hart, Ronald Philip, Sheldon, Michael H.
Format: eBook
Language:English
Published: San Rafael, Calif. (1537 Fourth Street, San Rafael, CA 94901 USA) : Morgan and Claypool, [2012]
Series:Colloquium digital library of life sciences.
Colloquium series on stem cell biology ; # 2.
Subjects:
Biobanks.
Fibroblasts.
Lymphocytes.
Mental illness > Genetic aspects.
Stem cells.
Biological Specimen Banks.
Mental Disorders > genetics.
Stem Cells.
fibroblasts
genetics
iPSC
lymphocytes
mental health
pluripotency
protocols
reprogramming
viral vectors
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Acknowledgments
  • 1. Introduction
  • 1.1 iPSC for the study of mental health disorders
  • 1.2 Building upon a robust genetics repository
  • 1.3 Protocols summary
  • 1.4 Outlook for the future of iPSC biobanking
  • 2. Appendix
  • 2.1 Mouse embryonic fibroblast (mEF) preparation
  • 2.2 Preparing irradiated mEF plates
  • 2.3 Fibroblast conversion
  • 2.4 PBMC conversion
  • 2.5 iPSC colony identification and selection
  • 2.6 iPSC expansion
  • 3. General protocols
  • 3.1 Equipment list
  • 3.2 Media list
  • 3.3 Preparation of gelatin-coated plates
  • 3.4 Preparation of BD Matrigel-coated plates
  • 3.5 Hemocytometer counting
  • 3.6 Manual scraping tool
  • 3.7 Identification and manual removal of differentiation
  • 3.8 Live alkaline phosphatase staining of iPSC
  • 3.9 Dispase dissociation of iPSC
  • 3.10 Accutase dissociation of iPSC
  • 3.11 Heat inactivation of fetal bovine serum
  • 3.12 Thawing and aliquoting sendai virus
  • References
  • Author biographies.