rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Characterization of Integrins and Osteopontin at the Uterine-Placental Interface During Pregnancy in Pigs and Sheep
  name:
    name part: Frank, James William
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Johnson, Gregory A.
    role:
      role term: thesis advisor.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2015
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (x, 156 pages) : illustrations
  abstract: Establishment of a successful pregnancy is dependent upon proper communication between the conceptus and uterus and provides the critical physiological and anatomical groundwork required to maintain growth and development of the conceptus throughout pregnancy. Osteopontin (OPN) is a soluble extracellular matrix (ECM) protein that interacts with multiple integrin receptors. Integrins are integral membrane proteins that promote cell-to-cell and cell-to-ECM adhesion, cytoskeletal reorganization, and signal transduction through a structure known as a focal adhesion (FA). Osteopontin, and multiple integrins that bind OPN, are observed on the apical surfaces of both trophectoderm (Tr) and uterine luminal epithelium (LE) during pregnancy in pigs and sheep. During the period of implantation in these species, conceptuses elongate and attach to the uterine LE. Placentation creates a unique physiological phenomenon wherein the apical surfaces of two epithelia adhere to one another for an extended period of time. Research for this dissertation investigated interactions between integrins and OPN at this unique interface. We hypothesized that FAs form in response to mechanical stress at the uterine-placental interface and utilize OPN as a bridging ligand to anchor placenta and uterine LE together in pigs and sheep during pregnancy. In the pig ITGAV and ITGB3 subunit proteins aggregate in vivo to form FAs during the peri-implantation period of pregnancy. As the uterine-placental interface becomes more folded during placentation, these FAs are no longer observed. We hypothesize that FAs are no longer necessary because folding disperses forces exerted across this interface. In the sheep, FAs containing integrins ITGAV, ITGA4, ITGA5, ITGB1, and ITGB5, which co-localize with OPN, increase in quantity and organization at the uterine-placental interface as pregnancy progresses. However, ITGB3 is only present during the peri-implantation period of pregnancy. The in vivo knockdown of ITGB…
  note: by James William Frank.
  note: "Major Subject: Biomedical Sciences"
  note: Includes vita.
  note: Doctor of Philosophy Texas A & M University 2015 http://hdl.handle.net/1969.1/155153
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Biomedical Sciences
  subject:
    topic: Osteopontin
  subject:
    topic: Integrin
  subject:
    topic: Focal Adhesion
  subject:
    topic: Implantation
  subject:
    topic: Pregnancy
  subject:
    topic: Pig
  subject:
    topic: Sheep
  identifier: http://hdl.handle.net/1969.1/155153
  record info:
    record content source: TXA
    record creation date: 160304
    record change date: 20170928112101.0
    record identifier: in00003614356
    language of cataloging:
      language term: eng