Protocols for Neural Cell Culture /

This second edition of a best-selling and highly praised collection of unfailingly reproducible methods includes key techniques to study the development and differentiation of the nervous system, new methods for isolating and propagating progenitor cells that can be stimulated to differentiate into...

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Bibliographic Details
Main Author: Fedoroff, Sergey
Corporate Author: SpringerLink (Online service)
Other Authors: Richardson, Arleen
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint : Humana Press, 1997.
Edition:Second edition.
Subjects:
Online Access:Connect to the full text of this electronic book
Description
Summary:This second edition of a best-selling and highly praised collection of unfailingly reproducible methods includes key techniques to study the development and differentiation of the nervous system, new methods for isolating and propagating progenitor cells that can be stimulated to differentiate into either neurons or various types of glial cells, and timely procedures for eliminating contaminating neural or nonneural cells. Important new procedures for the transfection of cells in culture with nontoxic mutant herpes simplex virus are also included, as are essential procedures for quantification, growth assays, evaluating neuroactive agents, preparing neural material for electron microscope analysis, and dissection. This book's unique wealth of practical detail and its inclusion of a wide range of tissue culture systems having many applications ensure that it will remain "a valuable resource that will find its place on many laboratory shelves (Trends in Biotechnology)."
Item Description:Electronic resource.
Physical Description:1 online resource (xx, 278 pages)
ISBN:9781475725865 (electronic bk.)
1475725868 (electronic bk.)