Imaging Living Cells /

Improved technology for imaging living cells, specific cellular targets and organelles is having a dramatic impact on basic and applied research. By combining optical design and molecular genetics, a new series of tools is being developed and successfully applied together with classical probes. Nove...

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Bibliographic Details
Main Author: Rizzuto, Rosario
Corporate Author: SpringerLink (Online service)
Other Authors: Fasolato, Cristina
Format: eBook
Language:English
Published: Berlin, Heidelberg : Springer Berlin Heidelberg, 1999.
Series:Springer lab manual.
Subjects:
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Strategies for Quantitative Digital Imaging of Biological Activity with Ion-Sensitive Fluorescent Probes
  • High Resolution 3-D Imaging by Image Restoration
  • Imaging Calcium in the Cytoplasm and in Organelles with Fluorescent Dyes
  • Confocal Calcium Imaging
  • Imaging Whole Organs
  • Simultaneous Measurement of Membrane Current and Intracellular Calcium.-Quantitative Calcium Imaging in Brain Slices
  • Common Methods for Measuring Apoptotic Cell Death with Flow Cytometry
  • Expressing, Targeting, and Calibrating Recombinant Aequorin
  • Analysis of Regulated Gene Expression by Microinjection and Digital Luminescence Imaging of Single Living Cells
  • The New Green Fluorescent Protein Mutants and Their Applications
  • Imaging Green Fluorescent Protein in Transgenic Plants.