http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
1 online resource (viii, 259 pages 91 illustrations)
This is a unique book that describes the most recent achievements in the methodology of protein biosynthesis under cell-free conditions. Various versions of cell-free protein-synthesizing systems and their applications to production of individual proteins on a preparative scale are reviewed. The most recent, advanced methodologies, such as continuous-exchange and continuous-flow cell-free systems and novel effecting batch-format cell-free procedures, are considered. Special attention is drawn to the possibilities of structural (NMR; X-ray) analysis of various gene expression products with the use of a new generation of cell-free systems.
From the contents: Cell-Free Protein Synthesis -- New Cell-Free Translation System Reconstructed with Purified Components -- Yeast Cell-Free Translation Systems -- Continuous-Flow and Continuous-Exchange Cell-Free Translation Systems and Reactors -- Co-Translational Protein Folding in Prokaryotic and Eukaryotic Cell-Free Translation Systems -- Structural and Functional Compensation by Proteins for the RNA Deficit of Animal Mitochondrial Translation Systems -- High Level Cell-Free Protein Expression from PCR Generated DNA-Templates -- Matrix Reactor: A New Scalable Reactor Principle for Cell-Free Protein Expression -- Selective Labeling of Proteins in the RTS 500 System -- Expression of Soluble Murine Endostatin with the RTS System -- Cell-Free Expression of Soluble Human Erythropoietin -- Cell-Free Expression of a 127 KD Protein: The Catalytic Subunit of Human Telomerase.