Plant tissue culture manual : Supplement 7 /
The Plant Tissue Culture Manual comprises a broad range of techniques of enormous value to research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering. Both applied and more fundamental uses of cell, tissue and organ culture are c...
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| Format: | eBook |
| Language: | English |
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New York :
Springer Science+Business Media,
1997.
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| Online Access: | Connect to the full text of this electronic book |
| Summary: | The Plant Tissue Culture Manual comprises a broad range of techniques of enormous value to research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering. Both applied and more fundamental uses of cell, tissue and organ culture are covered, and the ring-binder design allows the manual to be updated regularly with supplementary chapters. Each chapter, discussing a single technique or protocol, is written by an authority in the field and provides background information, references to the relevant literature and a guide to troubleshooting in addition to a thorough methodology. This Seventh Supplement to the Plant Tissue Culture Manual expands the existing sections: E: Reproductive Tissues; and Section H: Tissue Culture Techniques for Fundamental Studies. |
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| Item Description: | Originally published by Kluwer Academic Publishers. "Softcover reprint of the hardcover 1st edition 1997"--Title page verso. The manual is published initially as a core text of basic techniques, to which will be added supplementary chapters and sections at regular intervals. One characteristic of plant tissue culture methodology is the fact that what works for one species quite possibly will not work for another (or even for a different genotype of the same species): each requires a carefully optimized protocol, whether it be for callus culture initiation, protoplast isolation and culture or plant regeneration following Agrobacterium-mediated transformation. With this in mind, we have decided on the one hand to generalize experimental approaches for some basic techniques, by reference to the use of model species. This should at least give the researcher a feel for the techniques, and indeed provide a starting point for species-specific optimization of protocols. On the other hand, we have provided detailed protocols for the manipulation of a number of specific crop species, which have been difficult to optimize and for which there may be particular interest. A detailed bibliography is provided in each chapter, so that access to the relevant literature is possible. |
| Physical Description: | 1 online resource : illustrations |
| ISBN: | 9789400901032 (electronic bk.) 9400901038 (electronic bk.) |