rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Resin Microscopy and On-Section Immunocytochemistry
  name:
    name part: Newman, Geoffrey R.
    role:
      role term: creator
  name:
    name part: Hobot, Jan A.
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  genre: Electronic books.
  origin info:
    place:
      place term: gw
    date issued: 2001
    edition: Second edition.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (XX, 276 pages 38 illustrations)
  abstract: Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired and appropriate choices made. In the first part of the book, the background of the various resins available are discussed and information on inexpensive alternative technologies where they exist is provided. The various steps involved in tissue processing, beginning with fixation, are first described in theory, then detailed protocols are presented for their application, including troubleshooting sections. The second part rationalises the great variety of labelling methods that are commonly used for "on-section" cytochemistry and immunocytochemistry, where colloidal gold is currently overwhelmingly the most popular marker. The principles behind the usage of various markers and their limitations and advantages are discussed.
  note: by Geoffrey R. Newman, Jan A. Hobot.
  note: Electronic resource.
  subject:
    topic: Life sciences
  subject:
    topic: Immunology
  subject:
    topic: Biochemistry
  subject:
    topic: Cytology
  subject:
    topic: Biochemistry
  subject:
    topic: Cytology
  subject:
    topic: Immunology
  subject:
    topic: Life sciences
  classification: QH573-671
  classification: 571.6
  related item:
    title info:
      title: Springer lab manual
  identifier: 9783642569302 (electronic bk.)
  identifier: 3642569307 (electronic bk.)
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: I9W
    record creation date: 121227
    record change date: 20260422225427.2
    record identifier: in00003555400
    language of cataloging:
      language term: eng