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Techniques in prion research /

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This book provides the general description as well as the details of the techniques currently used for the study of prion diseases. Taking into account the pivotal role played by PrP it is not surprising that many Chapters of this book deal with the purification, the detection and the characterizati...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Lehmann, Sylvain, 1965-, Grassi, Jacques, 1951-
Format: eBook
Language:English
Published: Basel ; Boston : Birkhäuser, [2004]
Series:Methods and tools in biosciences and medicine.
Subjects:
Prions > Laboratory manuals.
Prion diseases > Laboratory manuals.
Prions > isolation & purification > Laboratory Manuals.
Prions > pathogenicity > Laboratory Manuals.
Disease Models, Animal > Laboratory Manuals.
Immunohistochemistry > methods > Laboratory Manuals.
Prion Diseases > etiology > Laboratory Manuals.
Prions (Virologie) > Manuels de laboratoire.
Maladies à prions > Manuels de laboratoire.
Prion > Forschungsmethode.
Prion diseases.
Prions.
Electronic books.
Aufsatzsammlung.
Handbooks, manuals, etc.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • 1 Transmissible Spongiform Encephalopathies or Prion Diseases - General Introduction
  • 2 Purification of PrPC
  • 3 Purification of the Pathological Isoform of Prion Protein (PrPSc or Prpres) from Transmissible Spongiform Encephalopathy-affected Brain Tissue
  • 4 Rapid Procedure for Purification and Renaturation of Recombinant PrP Protein
  • 5 Animal Models of Transmissible Spongiform Encephalopathies: Experimental Infection, Observation and Tissue Collection
  • 6 Cell Culture Models of TSEs
  • 7 PrPSc Immunohistochemistry
  • 8 Western Immunoblotting Techniques for the Study of Transmissible Spongiform Encephalopathies
  • 9 Characterization of Anti-PrP Antibodies and Measurement of PrP using ELISA Techniques
  • 10 TSE Strain Typing in Mice
  • 11 Biosafety and Decontamination Procedures
  • 12 Cell-Free Conversion of Prion Proteins
  • 13 Cytotoxicity of PrP Peptides
  • 14 Cyclic Amplification of Prion Protein Misfolding
  • Guide to Solutions
  • Guide to Protocols
  • Troubleshooting Guide.