FISH Technology /

Fluorescence in situ hybridization (FISH) is a powerful method to directly visualise the localisation of genomic alterations in the nucleus. The technique has been adapted to a wide range of applications in both medicine, especially diagnostic cytogenetics, and biology. Topics described in this manu...

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Bibliographic Details
Main Author: Rautenstrauss, Bernd W.
Corporate Author: SpringerLink (Online service)
Other Authors: Liehr, Thomas
Format: eBook
Language:English
Published: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint : Springer, 2002.
Series:Springer lab manual.
Subjects:
Online Access:Connect to the full text of this electronic book
Description
Summary:Fluorescence in situ hybridization (FISH) is a powerful method to directly visualise the localisation of genomic alterations in the nucleus. The technique has been adapted to a wide range of applications in both medicine, especially diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, e.g. formalin-fixed and paraffin-embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and expression of apoptosis-related genes; comparative genomic hybridization; special FISH techniques (Fiber-, PNA-, CO-, NU- and DBD-FISH); FISH on insect cells and yeast; multi-color FISH applications (spectral karyotyping analysis, reverse FISH and FICTION); FISH-related techniques, such as PRINS and microarrays.
Item Description:Electronic resource.
Physical Description:1 online resource (x, 494 pages 78 illustrations, 44 illustrations in color.)
ISBN:9783642564048 (electronic bk.)
3642564046 (electronic bk.)