Flow cytometry and cell sorting /
The practical aspects of flow cytometry and sorting are emphasized in this book which introduces the beginner to the technology and provides tips and tricks for the advanced user. The clear structure makes it easy to address specific problems fast. The chapters cover the modern applications of these...
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| Format: | eBook |
| Language: | English |
| Published: |
Berlin ; New York :
Springer-Verlag,
[1992]
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| Series: | Springer laboratory.
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| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Operation of a flow cytometer
- Conjugation of fluorochromes, haptens, and phycobiliproteins to antibodies
- Immunofluorescence : basic considerations
- Multicolor immunofluorescence analysis
- Combined intracellular and surface staining
- Scatchard analysis by flow cytometry
- Preparation and staining of cells for high-resolution DNA analysis
- Simultaneous flow cytometric detection of bromodeoxyuridine incorporation and cell surface marker expression
- High-resolution cell cycle analysis : the Flow Cytometric Bromodeoxyuridine
- Hoechst Quenching Technique
- Cell activation : Indo-1 ratiometric analysis of intracellular ionized calcium
- Biochemical parameters of cell function
- Molecular analysis of transcriptional control : the FACS-Galactosidase assay
- Ligand acidification by nonadherent cells
- Powerful preselection
- High gradient magnetic cell
- Sorting
- Setup of a flow sorter
- Sorting of rare cells
- Fluorescence-activated chromosome sorting
- Flow cytometry and sorting of plant chromosomes
- Large particle sorting
- Biological and laser safety.