Quantitation of mRNA by Polymerase Chain Reaction : Nonradioactive PCR Methods /
In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques for quantifying nucleic acids in biological and clincial samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also...
| Main Author: | |
|---|---|
| Corporate Author: | |
| Other Authors: | , , , , |
| Format: | eBook |
| Language: | English |
| Published: |
Berlin, Heidelberg :
Springer Berlin Heidelberg,
1996.
|
| Series: | Springer Labor Manuals.
|
| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
| Summary: | In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques for quantifying nucleic acids in biological and clincial samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quanatitative PCR protocols into their own clinical or biomedical research. |
|---|---|
| Item Description: | Electronic resource. |
| Physical Description: | 1 online resource (XX, 166 pages 15 illustrations) |
| ISBN: | 9783642797125 (electronic bk.) 3642797121 (electronic bk.) |