rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Yeast Genetics
    sub title: a Manual of Methods
  name:
    name part: Spencer, J. F. T.
    role:
      role term: creator
  name:
    name part: Spencer, Dorothy M.
  name:
    name part: Bruce, I. J.
  name:
    name part: SpringerLink (Online service)
  type of resource: text
  genre: Electronic books.
  origin info:
    place:
      place term: gw
    date issued: 1989
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (VII, 104 pages 10 illustrations)
  abstract: This book is designed for use as a manual of methods, to be used at the bench. Topics discussed and methods described include classical genetics as related to yeasts, such as mating, sporulation, isolation of hybrids, microdissection of asci or the isolation of single-spore clones, mapping of genes and the construction of new strains by protoplast fusion. There is a large section dealing with mutations in general, and with methods of isolating a number of important classes of mutants in particular. A second part deals with basic techniques in separation of chromosomes by electrophoresis, such as OFAGE, FIGE, and CHEF, and gives detailed protocols for the first two. This section further includes methods for isolation of high molecular weight DNA from yeast, isolation of RNA, and techniques for transformation of yeasts.
  table of contents: Contents: Introduction -- "Classical" Yeast Genetics: Mating. Sporulation. Tetrad Analysis. Enzymes for Digestion of Yeast Ascus Walls. Ancillary Methods for Handling Clones Obtained by Ascus Dissection. Mutagenesis. Mapping and Fusion. Protoplast Formation and Fusion -- Methods Using Direct Manipulation of DNA and RNA: Separation of Large DNA Molecules, Greater than 25 Kbp, by Gel Electrophoresis. Isolation of Pure High-Molecular-Weight DNA from the Yeast Saccharomyces cerevisiae. Transformation of Yeast: Saccharomyces cerevisiae. Plasmid Isolation from Yeast. Rapid Isolation of DNA from Yeast. RNA Isolation -- Subject Index.
  note: by John F.T. Spencer, Dorothy M. Spencer, I.J. Bruce.
  note: Electronic resource.
  subject:
    topic: Life sciences
  subject:
    topic: Biotechnology
  subject:
    topic: Biochemistry
  subject:
    topic: Cytology
  subject:
    topic: Biochemistry
  subject:
    topic: Biotechnology
  subject:
    topic: Cytology
  subject:
    topic: Life sciences
  classification: QH573-671
  classification: 571.6
  identifier: 9783642733567 (electronic bk.)
  identifier: 3642733565 (electronic bk.)
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: I9W
    record creation date: 121227
    record change date: 20260422170346.2
    record identifier: in00003543465
    language of cataloging:
      language term: eng