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Rapid cycle real-time PCR : methods and applications : genetics and oncology /

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Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products wi...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Dietmaier, W. (Wolfgang), 1963-, Wittwer, C. (Carl), 1955-, Sivasubramanian, N. (Natarajan)
Format: eBook
Language:English
Published: Berlin ; New York : Springer, [2002]
Subjects:
Polymerase chain reaction.
Molecular genetics > Methodology.
Cancer > Genetic aspects.
Molecular probes.
Molecular Diagnostic Techniques.
Genetic Diseases, Inborn > diagnosis.
Molecular Probes.
Neoplasms > diagnosis.
Polymerase Chain Reaction > methods.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • From the contents: Rapid Genotyping of 2-bp and 9-bp Deletion Mutations by LightCycler
  • Rapid Screening for Five Major Cystic Fibrosis Mutations by Melting Peak Analysis Using Fluorogenic Hybridization Probes
  • Genotyping of the Methionnine-Valine Polymorphism at Codon 129 of the Human Prion Protein by Melting Point Analysis of Fluorescently Labeled Hybridization Probes
  • SYBR Green I Genotyping of Trinucleotide Repeat Loci by Tm
  • Limitations of Melting Curve Analysis Using SYBR Green I
  • Fragment Differentiation and Mutation Detection in the CFTR-Gene
  • Parallel Genotyping of Different Genes: A Rapid Real-Time PCR Approach
  • Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene
  • Rapid Quantitative Detection of Free Cancer Cells in the Peritoneal Cavity of Gastric Cancer Patients with Real-Time CEA RT-PCR Using Hybridization Probes
  • Expression Analysis of Telomerase-Genes hTERT and hTR by quantitative PCR on the LightCycler.