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Rapid cycle real-time PCR : methods and applications : genetics and oncology /

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Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products wi...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Dietmaier, W. (Wolfgang), 1963-, Wittwer, C. (Carl), 1955-, Sivasubramanian, N. (Natarajan)
Format: eBook
Language:English
Published: Berlin ; New York : Springer, [2002]
Subjects:
Polymerase chain reaction.
Molecular genetics > Methodology.
Cancer > Genetic aspects.
Molecular probes.
Molecular Diagnostic Techniques.
Genetic Diseases, Inborn > diagnosis.
Molecular Probes.
Neoplasms > diagnosis.
Polymerase Chain Reaction > methods.
Electronic books.
Online Access:Connect to the full text of this electronic book
Description
Summary:Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I allow product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms. The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and analysis in the fields of Genetics and Oncology. Molecular analysis has never been easier!
Physical Description:1 online resource (viii, 205 pages) : illustrations (some color)
Bibliography:Includes bibliographical references.
ISBN:9783642593970 (electronic bk.)
3642593976 (electronic bk.)