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Techniques in molecular medicine /

To date, molecular biology techniques have been introduced to all fields of modern biomedical research and are now finding their way into diagnosis and even therapy. This laboratory manual is designed for researchers who have started or are planning to use molecular biology techniques and need relia...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Hildebrande, Friedhelm, 1957-, Igarashi, Peter
Format: eBook
Language:English
Published: Berlin ; New York : Springer, [1999]
Series:Springer lab manual.
Subjects:
Molecular biology > Laboratory manuals.
Molecular diagnosis > Laboratory manuals.
Medical genetics > Laboratory manuals.
Molecular Biology > methods > Laboratory Manuals.
Moleculaire biologie.
Laboratoriumonderzoek.
Medical genetics.
Molecular biology.
Molecular diagnosis.
Electronic books.
Handbooks, manuals, etc.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Structure and function of the human genome / Friedhelm Hildebrandt
  • Enzymatic modification of DNA and RNA / Kai-Olaf Netzer
  • Purification and nucleic acids from eukaryotic cells / Hans Gerd Nothwang and Friedhelm Hildebrandt
  • Electrophoresis of DNA and RNA fragments / Feng Qian and Gregory G. Germino
  • Single-strand conformation polymorphism (SSCP) analysis / Feng Qian and Gregory G. Germino
  • Denaturing gradient gel electrophoresis / Kai-Olaf Netzer
  • Introduction of pulsed-field gel electrophoresis (PFGE) / Feng Qian and Gregory G. Germino
  • Pulsed-field gel electrophoresis : protocols / Gudrun A. Rappold [and others]
  • Radioactive labeling of DNA and RNA probes / Peter Igarashi
  • Hybridization methods (southern and northern blotting) / Kai-Olaf Netzer
  • Specimen preparation techniques for cytogenic analysis by fluorescence in situ hybridization / Stefan Joos [and others]
  • Chromosome analysis by fluorescence in situ hybridization / Martin Bentz, Stefan Joos and Peter Lichter
  • Sequencing / Klaus Deichmann
  • Polymerase chain reaction / Friedhelm Hildebrandt and Iva Singh-Sawhney
  • Cloning vectors / Annette Deichmann and Klaus Deichmann
  • Cloning strategies : an overview / Friedhelm Hildebrandt
  • Genomic libraries / Luitz F. Onuchic and Gregory G. Germino
  • cDNA libraries / Luitz F. Onuchic and Gregory G. Germino
  • cDNA library screening / Johann Peter Hossle
  • Expression cloning with Xenopus oocytes / Daniel Markovich, Andreas Werner and Heini Murer
  • Transgenic animals / Eckart Schott, Martin Paul and Detlev Ganten
  • Expression in eukaryotic cells : gene transfer using retroviral vectors / Martin Marx
  • Positional cloning and linkage analysis / Friedhelm Hildebrandt and Heymut Omran
  • Protein techniques / Robert F. Reilly.