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Basic cloning procedures /

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This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Berzins, Valdis, 1943-
Format: eBook
Language:English
Published: Berlin ; New York : Springer, [1998]
Series:Springer lab manual.
Subjects:
Molecular cloning > Laboratory manuals.
Cloning, Molecular > Laboratory Manuals.
Molecular cloning.
Kloneren.
Wetenschappelijke technieken.
Genklonierung.
Molekularbiologie.
Electronic books.
Handbooks, manuals, etc.
Online Access:Connect to the full text of this electronic book
Description
Summary:This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.
Item Description:Electronic resource.
Physical Description:1 online resource (169 pages) : illustrations.
Format:Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Bibliography:Includes bibliographical references and index.
ISBN:9783642719653 (electronic bk.)
3642719651 (electronic bk.)