PCR topics : usage of polymerase chain reaction in genetic and infectious diseases /
The Polymerase chain reaction (PCR) has revolutionized the technical development of molecular biology and immunology. It is a very simple method for amplifying nucleic acids in vitro. During the last two years PCR has mostly been used on investigations of oncogenes, tumor associated translocations,...
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| Format: | eBook |
| Language: | English |
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Berlin ; New York :
Springer-Verlag,
[1991]
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| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Basic Methodology and Research Applications
- Quantitation of mRNA by the Polymerase Chain Reaction
- Sequencing of PCR Products Analysis of Factor IX Genes and of Recombination Events in Immunoglobulin Genes
- Alternative Methods for DNA Probing in Diagnosis: Ligase Chain Reaction (LCR)
- Application of PCR to the Detection and Analysis of Point Mutations in the Human Factor VIII Gene
- The Use of PCR in the Diagnosis of Muscle Disease
- Taq DNA Polymerase-Synthesized Single-Stranded DNA Hybridization Probes and their Application in Northern Blotting and in situ Hybridization
- Computer Program for the Selection of PCR Primers
- Nervous System Cross Reactivity Sequences
- The Use of Short Adapters for Priming PCR of Unknown Chromosomal DNA Fragments
- Labeling of Specific DNA Sequences With Digoxigenin during Polymerase Chain Reaction
- Quantitative Analysis of Polymerase Chain Reaction (PCR) Products by Means of Fluorescence Detection
- Reverse Transcription PCR (RT-PCR) of Parathyroid Hormone (PTH) mRNA
- False Positive Reactions in PCR
- Error Production and Error Propagation During PCR
- Genetics and Evolution
- PCR in Linkage Analysis of Genetic Diseases
- Amplification, Isolation and Direct Nucleotide Determination of Entire Genes: Application to the Study of 16S rRNAs for Molecular Evolution in Bacteria, Identification of Cultural Isolates and Development of Probes
- Diagnosis of Cystic Fibrosis Detection of the Gene Deletion Delta F 508
- Application of PCR for Prenatal Diagnosis of Cystic Fibrosis (CF)
- The Use of Non-Viable Cultured Amniotic Fluid Cells for Prenatal Diagnosis of Cystic Fibrosis by Polymerase Chain Reaction
- Determination of Glutathione S-Transferase u Genotype in Humans by PCR-Amplified Gene-Probes
- Rapid Apolipoprotein E Genotype Identification
- Detection and Characterization of Viral Infections
- Herpes Viruses
- Detection of Herpes Viruses by PCR Diagnostic Aspects
- Diagnosis of Herpes Simplex Virus Encephalitis (HSVE) by Polymerase Chain Reaction
- Rapid Detection of CMV-Specific DNA and mRNA by PCR in Immunocompromised Patients
- Specific and Sensitive Detection of Cytomegalovirus DNA in Clinical Specimens by PCR
- Detection of EBNA2-mRNA in Primary Central Nervous System Lymphomas in Patients with AIDS
- Detection of Epstein-Barr Virus Genomes in Lymphoproliferative Diseases
- Screening of Lymphatic Tissues for the Presence of Human Herpesvirus 6 DNA Using the Polymerase Chain Reaction (PCR)
- Retroviruses
- HTLV Retrovirus in Neurological Disease
- Use of PCR to Investigate the Cellular Tropism of Human T Cell Leukemia Virus Type I
- Viral Genome Sequences in Cerebrospinal Fluid and Serum of HIV-1 Infected but Asymptomatic Patients
- Hepatitis Viruses
- PCR Detection of HBV Replication in Anti-HBeAg Chronic Carriers with Pre-S Antigenemia
- Detection of Virus DNA by PCR in Hepatitis Non-A, Non-B
- Hepatitis B Virus DNA Identification by Polymerase Chain Reaction (PCR) and Correlation with Serological HBV Markers
- Detection and Characterization of Bacterial Infections
- Rapid Diagnosis of Mycobacterium Tuberculosis Infection by PCR
- Detection of Chlamydia Trachomatis by PCR
- The Polymerase Chain Reaction (PCR) to Detect Gene Sequences of Borrelia Burgdorferi, the Etiologic Agent of Lyme Disease
- Rapid Diagnosis of Human Listeriosis Using the Polymerase Chain Reaction
- Oncogenes
- Quantitation of Mdr1 Transcript by PCR a Tool for Monitoring Drug Resistance in Cancer Chemotherapy
- Ras Oncogene Mutations in Renal Cell Cancer
- Primer Mismatch Analysis: a Rapid and Non-Isotopic Method for Analysing Ras Mutations in Human Cancer
- Expression of Adenovirus Type 12 13S Gene Product is Sufficient to Immortalize Primary Mouse Cells
- PCR-Based Rapid Detection of the Philadelphia-Chromosome in Chronic Myelogenous and Acute Lymphoblastic Leukemia
- List of Contributors.