rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Regulation of DNA Replication During Conventional and Unconventional Cell Cycles in Tetrahymena
  name:
    name part: Lee, Po-Hsuen
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Kapler, Geoffrey M.
    role:
      role term: thesis advisor.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2015
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: As the nucleating protein for pre-replicative complex (pre-RC) assembly, the conserved Origin Recognition Complex (ORC) specifies where replication initiates in eukaryotic chromosomes. During the vegetative cell cycle of Tetrahymena thermophila, previously published work has shown that DNA replication initiates from defined chromosomal sites in an ORC-dependent manner. Tetrahymena exhibits nuclear dimorphism, a polyploid somatic macronucleus (MAC), which is transcriptionally active and maintains vegetative growth, and a diploid germline micronucleus (MIC) responsible for the transmission of genetic information during conjugation. In order to provide more information about the fundamental mechanisms of micro- and macro-nuclear replication programs, I study the impacts of changing in ORC protein contents on the fate of micro- and macro-nuclear chromosomes during the vegetative cell cycle and development in Tetrahymena. I examined the effect of down-regulation of ORC1 on genome stability and intra-S phase checkpoint activation by disrupting ORC1 gene in the macronucleus. Partial depletion of Orc1p leads to genome instability in the diploid mitotic micronucleus, abnormal division of the polyploid amitotic macronucleus, and failure to mount a robust intra-S phase checkpoint response. In addition, the ORC1 knockdown strain fails to execute two developmentally-regulated DNA replication programs, endoreplication and ribosomal DNA (rDNA) gene amplification. I also examined the regulation of ORC and MCM during development. Remarkably, the result suggests that the demand on the ORC-dependent replication machinery differs during development and the vegetative S phase. To further gain new insights into fundamental mechanisms that protect chromosomes from replication stress, I examined the impact of replication stress on the regulation of ORC and MCM. This study led to the discovery of a novel DNA replication program that is activated under HU treatment. While Orc1p and Mcm6p wer…
  note: by Po-Hsuen Lee.
  note: "Major Subject: Medical Sciences"
  note: Includes vita.
  note: Doctor of Philosophy Texas A & M University 2014 http://hdl.handle.net/1969.1/152509
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Medical Sciences
  subject:
    topic: cell cycle
  subject:
    topic: intra-S checkpoint
  subject:
    topic: DNA replication
  subject:
    topic: Origin Recognition Complex (ORC)
  subject:
    topic: Tetrahymena
  identifier: http://hdl.handle.net/1969.1/152509
  record info:
    record content source: TXA
    record creation date: 150218
    record change date: 20151206125926.0
    record identifier: in00003496806
    language of cataloging:
      language term: eng