rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Application of Stable Isotope Probing to Identify RDX-degrading Bacteria in Groundwater
  name:
    name part: Cho, Kun-Ching
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Chu, Kung-Hui
    role:
      role term: thesis advisor.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2014
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is soluble, nonvolatile cyclic nitramine explosive. Long-term manufacturing and various applications of RDX have resulted in RDX contamination in soil and groundwater. RDX is a possible human carcinogen; therefore, occurrence of RDX in groundwater has raised a public health concern. As RDX is biodegradable; bioremediation of RDX-contaminated groundwater has been recognized as a feasible cleanup technology. Several RDX-degrading isolates are known to have ability to utilize RDX as carbon and/or nitrogen source. However, little is known about these isolates and their roles in the natural or engineered systems during RDX degradation, or about RDX-degrading microbial communities in responding to engineered interventions. Stable isotope probing (SIP) is a powerful culture independent method that can identify functional active bacteria in various environmental samples. In this study, we applied SIP with ¹³C-labeled or one of the ring-, nitro- and fully-labeled ¹⁵N-RDX to identify microorganisms capable of utilizing RDX and its metabolites as carbon and/or nitrogen sources in groundwater microcosms, and to associate active RDX-degrading microbial communities in responding to engineered interventions. Derived sequences from ¹³C-DNA were clustered in Bacteroidia, Clostridia, α-, β- and δ-Proteobacteria, and Spirochaetes, which were different from previously described RDX degraders. Cheese whey amendment stimulated RDX biotransformation, altered the types of RDX-degrading bacteria, and decreased microbial community diversity. Derived sequences from ¹⁵N-DNA were grouped in Clostridia, β-Proteobacteria and Spirochaetes. In comparison to the results among ¹³C-SIP and ¹⁵N-SIP studies with presence of cheese whey, derived sequences were clustered in γ-Proteobacteria and Bacilli. The combination of these findings suggested that RDX-degrading microorganisms in groundwater are more phylogenetically diverse than what has been inferred from…
  note: by Kun-Ching Cho.
  note: "Major Subject: Civil Engineering"
  note: Includes vita.
  note: Doctor of Philosophy Texas A & M University 2013 http://hdl.handle.net/1969.1/151877
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Civil Engineering
  subject:
    topic: Stable isotope probing
  subject:
    topic: RDX
  identifier: http://hdl.handle.net/1969.1/151877
  record info:
    record content source: TXA
    record creation date: 140627
    record change date: 20151206101910.0
    record identifier: in00003483387
    language of cataloging:
      language term: eng