rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    non sort: The
    title: Impact of Extracellular Matrix Stiffness on Angiogensis
  name:
    name part: Lee, Po-Feng
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Yeh, Alvin
    role:
      role term: thesis advisor.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2013
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: Sprouting endothelial cells (ECs) use soluble and insoluble cues to guide migration and expand the existing vascular network to meet changing trophic needs of the tissue during angiogenesis. A noninvasive and non-destructive nonlinear optical microscopy (NLOM) technique was used to optically image endothelial sprouting morphogenesis in three dimensional (3D) collagen matrices with simultaneously captured signals from collagen fibers and endothelial cells using second harmonic generation (SHG) and two-photon excited fluorescence (TPF), respectively. Sprout advancement and lumen expansion companying with ECM alteration were the synergistic results of membrane-associated matrix metalloproteinase and cell traction evidenced by proteinase inhibition and Rho-associated kinase (p160ROCK) inhibition experiments. These physical EC-ECM interactions suggest that ECM mechanical properties may influence angiogenic responses. In a 3D angiogenesis model, we measure angiogenic responses as a function of collagen matrix stiffness by inducing collagen cross-linking with microbial transglutaminase (mTG). Collagen matrices stiffen with both mTG treatment and incubation time as evidenced with biaxial mechanical test results and collagen TPF intensity increases with mTG treatment and that the ratio of TPF/SHG correlates with biaxial tested mechanical stiffness. SHG and optical coherence microscopy (OCM) are further used to show that other physical properties of the matrix do not change with mTG treatment, thus providing the same density but different stiffness with which to measure angiogenic responses. Stiffer matrices promote angiogenesis with more invading sprouts that invade deeper. No differences in lumen size were observed between control and mTG stiffened 3D cultures, but there was evidence of greater matrix remodeling in stiffer gels using NLOM. Results of this study show angiogenic responses are enhanced with increasing stiffness and suggest that these properties may be used in …
  note: by Po-Feng Lee.
  note: "Major Subject: Biomedical Engineering"
  note: Includes vita.
  note: Doctor of Philosophy Texas A & M University 2012 http://hdl.handle.net/1969.1/148244
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Biomedical Engineering
  subject:
    topic: Invasion
  subject:
    topic: Microbial transglutaminase
  subject:
    topic: OCM
  subject:
    topic: Outgrowth
  subject:
    topic: 3D
  subject:
    topic: Collagen
  subject:
    topic: TPF
  subject:
    topic: SHG
  subject:
    topic: NLOM
  subject:
    topic: Angiogenesis
  subject:
    topic: ECM
  subject:
    topic: Endothelial
  identifier: http://hdl.handle.net/1969.1/148244
  record info:
    record content source: TXA
    record creation date: 140113
    record change date: 20151206090924.0
    record identifier: in00003431415
    language of cataloging:
      language term: eng