rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Exploring the Effects of Crosslinking on the Intervertebral Disc
  name:
    name part: Kirking, Bryan Clifford
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Criscione, John
    role:
      role term: thesis advisor.
  name:
    name part: Hedman, Tom
    role:
      role term: thesis advisor.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2013
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: Crosslinking soft tissue has become more common in tissue engineering applications, and recent studies have demonstrated that soft tissue mechanical behavior can be directly altered through crosslinking, but increased understanding of how crosslinking affects intervertebral disc mechanical behavior is needed. In vitro testing of bovine disc and motion segments was used to characterize several important aspects of disc behavior in response to crosslinking after both soaking and injection treatment. The first study was a comparison of different crosslinkers to determine the effect on tensile properties of disc tissue. Circumferential specimens were taken from bovine annulus and then soak treated with an optimized crosslinking formulation or sham solution. A non-contacting laser micrometer was used to measure cross sectional area, after which tension testing until failure was performed to determine yield strain, yield stress, ultimate stress, peak modulus, and resilience. The crosslinkers were observed to produce different changes in the properties, with the measured properties generally increasing. The second study used bilateral annular injections to simulate a clinically relevant delivery method. The dose response of the motion segment's neutral zone stability metrics against injection concentration was mapped. Concentrations of 20 mM and less had no significant effects on the stability metrics. 40mM demonstrated a change in neutral zone stiffness, while at least 80mM was required to significantly affect neutral zone length. Thus, meaningful changes in joint neutral zone stability were demonstrated using clinically relevant injection and chemical formulations. The third study used combinations of biochemical and accelerated mechanical cyclic loading to degrade gelatin and annulus fibrosus specimens with and without genipin treatment. Genipin crosslinking attenuated changes during cyclic loading to specimen geometry and compliance relative to control samples. Full re…
  note: by Bryan Clifford Kirking.
  note: "Major Subject: Biomedical Engineering"
  note: Includes vita.
  note: Doctor of Philosophy Texas A & M University 2012 http://hdl.handle.net/1969.1/148231
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Biomedical Engineering
  subject:
    topic: Spine
  subject:
    topic: Degradation
  subject:
    topic: Interfacial shear
  subject:
    topic: Neutral zone
  subject:
    topic: Tensile
  subject:
    topic: Circumferential
  subject:
    topic: Genipin
  subject:
    topic: Crosslinking
  subject:
    topic: Intervertebral disc
  identifier: http://hdl.handle.net/1969.1/148231
  record info:
    record content source: TXA
    record creation date: 140116
    record change date: 20151206091152.0
    record identifier: in00003431055
    language of cataloging:
      language term: eng