rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Hybrid Polyethylene Glycol Hydrogels for Tissue Engineering Applications
  name:
    name part: Munoz Pinto Dany Jair
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Hahn, Mariah S.
    role:
      role term: thesis advisor.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2013
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: Currently, organ transplant procedures are insufficient to address the needs of the number of patients that suffer of organ failure related disease. In the United States alone, only around 19% of the patients are able to get an organ transplant surgery and 25% die while waiting for a suitable donor. Tissue engineering (TE) has emerged as an alternative to organ transplant; thus, the aim of the present study was to validate a poly(ethylene glycol) diacrylate (PEG-DA) hydrogel system as a model for material scaffolding in TE applications. This work explores the influence of scaffold material properties on cell behavior. Specifically, scaffold modulus, mesh size, and biochemical stimuli were characterized and their influence on cell response was analyzed at the biochemical, histological and microenvironmental levels. Three different TE targets were evaluated: vocal fold restoration, vascular grafts and osteochondral applications. Vocal fold fibroblast (VFF) phenotype and extracellular matrix (ECM) production were impacted by initial scaffold mesh size and modulus. The results showed increasing levels of SM-α-actin and collagen production with decreasing initial mesh size/increasing initial modulus, which indicated that VFFs were induced to take an undesirable myofibroblast-like phenotype. In addition, it was possible to preserve VFF phenotype in long-term cultured hydrogels containing high molecular weight hyaluronan (HAHMW). On the other hand, regarding vascular graft applications, smooth muscle cell (SMC) phenotype was enhanced by increasing scaffold mesh size and modulus. Finally, the effect of scaffold inorganic content (siloxane) on rat osteoblasts and mouse mesenchymal stem cells was evaluated. Interestingly, the impact of inorganic content on cell differentiation seemed to be highly dependent on the initial cell state. Specifically, mature osteoblasts underwent transdifferentiation into chondrocyte-like cells with increasing inorganic content. However, Mesenchym…
  note: by Dany Jair Munoz Pinto.
  note: "Major Subject: Chemical Engineering"
  note: Includes vita.
  note: Doctor of Philosophy Texas A & M University 2011 http://hdl.handle.net/1969.1/149213
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Chemical Engineering
  subject:
    topic: Bone tissue engineering
  subject:
    topic: Vascular grafts
  subject:
    topic: Tissue engineering
  subject:
    topic: Hydrogel
  identifier: http://hdl.handle.net/1969.1/149213
  record info:
    record content source: TXA
    record creation date: 140127
    record change date: 20151206091321.0
    record identifier: in00003428745
    language of cataloging:
      language term: eng