rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Identification of Root-knot Nematode Resistance Loci in Gossypium hirsutum using Simple Sequence Repeats
  name:
    name part: Del Rio, Sonia Y
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Starr, James L.
    role:
      role term: thesis advisor.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2013
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: Gossypium hirsutum, upland cotton, is one of the major crops grown in the United States and the world. Upland cotton is cultivated in areas that are ideal breeding grounds for the difficult to manage, southern root-knot nematode (RKN), Meloidogyne incognita. Host plant resistance is the most effective way to control RKN populations. However, resistance used in most breeding programs stems from a few related sources. Novel sources of resistance have been identified but have yet to be introduced into elite breeding lines or genetically studied. The objectives of this study are two-fold. The first is to develop elite germplasm by introgressing RKN resistance from primitive accessions into modern cotton genotypes via backcrossing. The second is to use simple sequence repeats (SSRs) to identify loci associated with RKN resistance in the primitive accessions. The genotypes used will be: 1) inoculated with M. incognita, 2) phenotypically analyzed by measuring the nematode reproduction as eggs per gram of fresh root and host response using a root gall index, 3) genetically evaluated by using SSR markers to detect polymorphisms between the RKN resistant TX accessions and DP90 (susceptible genotype), and 4) analyzed using linkage and mapping software. Elite germplasm that contains: 1) high yield potential and a high level of RKN- resistance or 2) high fiber quality and RKN-resistance was developed by performing two backcrosses based on phenotypic analyses. A third screen is currently underway to ensure the introgression of the RKN resistance genes. Agronomic tests will need to be done before the germplasm is released. Genetic analyses using SSR-based primer sets of the TX accessions did not yield expected results. Of the 508 primers sets tested, only 31 were polymorphic between the TX accessions and DP90. A bulked segregant analysis approach was used to test the 31 primer sets on the resistant and susceptible bulks of the F2 population but no polymorphisms were seen. However,…
  note: by Sonia Y. Del Rio.
  note: "Major Subject: Plant Pathology"
  note: Includes vita.
  note: Doctor of Philosophy Texas A & M University 2013 http://hdl.handle.net/1969.1/149444
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Plant Pathology
  subject:
    topic: Meloidogyne incognita
  subject:
    topic: root-knot nematode
  subject:
    topic: Gossypium hirsutum
  subject:
    topic: upland cotton
  identifier: http://hdl.handle.net/1969.1/149444
  record info:
    record content source: TXA
    record creation date: 140123
    record change date: 20151206091320.0
    record identifier: in00003427229
    language of cataloging:
      language term: eng