rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    non sort: The
    title: Role of Docosahexaenoic Acid in Regulation of Epidermal Growth Factor Receptor Activation and Function
  name:
    name part: Turk, Harmony Faith
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Chapkin, Robert
    role:
      role term: thesis advisor.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2013
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: The epidermal growth factor receptor (EGFR) is a transmembrane receptor tyrosine kinase integral in regulating cell growth, survival, and migration. EGFR signaling, which is dependent on localization of the receptor within lipid rafts, is often hijacked during colon tumorigenesis. Previous work has found that docosahexaenoic acid (DHA) is protective against colon cancer. This fatty acid is proposed to function in part by pertubing lipid rafts and thereby altering cell signaling. The overall objective of this work was to determine whether DHA alters EGFR function and signaling. We assessed EGFR localization and ligand-induced phosphorylation in YAMC cells treated with fatty acids. We found that DHA reduced the localization of EGFR to lipid rafts. Concomitant with altering receptor localization, DHA was found to increase EGFR phosphorylation. However, DHA paradoxically suppressed EGFR signal transduction. We found that DHA uniquely altered EGFR activity, and other long chain polyunsaturated fatty acid did not exert the same effect. We additionally observed similar effects on EGFR activation and signaling by feeding mice a diet enriched in fish oil (high in DHA), and this was attendant with reduced colon tumorigenesis. We next probed the mechanism by which DHA enhances EGFR phosphorylation. We found that DHA facilitates receptor dimerization to increase phosphorylation. We additionally identified Ras activation as the site of perturbation of signal transduction. DHA suppressed signal transduction by both changing the localization of EGFR within the plasma membrane and increasing receptor endocytosis and degradation. Lastly, we extended our observations into a wounding model. Although DHA uniquely altered ligand-stimulated EGFR activity, both DHA and EPA altered EGFR transactivation and signaling upon injury. This culminated in reduced wound healing in DHA and EPA treated cells. In an animal model, we found that diets enriched in either DHA or EPA altered EGFR signaling…
  note: by Harmony Faith Turk.
  note: "Major Subject: Nutrition"
  note: Includes vita.
  note: Doctor of Philosophy Texas A & M University 2012 http://hdl.handle.net/1969.1/148103
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Nutrition
  subject:
    topic: signal transduction
  subject:
    topic: Ras
  subject:
    topic: colon cancer
  subject:
    topic: n-3 polyunsaturated fatty acid
  subject:
    topic: docosahexaenoic acid
  subject:
    topic: lipid raft
  subject:
    topic: epidermal growth factor receptor
  identifier: http://hdl.handle.net/1969.1/148103
  record info:
    record content source: TXA
    record creation date: 131219
    record change date: 20151206090654.0
    record identifier: in00003421207
    language of cataloging:
      language term: eng