rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Identification of ARGONAUTES involved in antiviral RNA silencing in Nicotiana benthamiana
  name:
    name part: Odokonyero, Denis
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Scholthof, Herman
    role:
      role term: thesis advisor.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2013
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (x, 163 pages) : illustrations
  abstract: ARGONAUTE proteins (AGOs) are generally accepted as key components of the post transcriptional gene silencing mechanism, also involved in plant antiviral defense. Except for reports on the antiviral roles of AGO1, AGO2 and AGO7 in Arabidopsis, the exact roles played by the individual AGOs in other plant species are largely unknown. This research focused on the identification and characterization of AGOs involved in antiviral RNAi response to various viruses in N. benthamiana. Based on the temporal and spatial distribution of AGO transcripts in 3 and 8-week old plant root, stem and leaf tissues, expressions of NbAGO mRNAs were found to vary with age and tissue specificity. Plant endogenous AGO mRNAs were knocked down through virus induced gene silencing techniques using the Tobacco rattle virus vector system and posteriorly challenged with a GFP-chimeric virus construct deficient of a silencing suppressor. Unlike in control non-silenced plants, the Tomato bushy stunt virus construct deficient of its P19 silencing suppressor was consistently seen to exhibit a strong fluorescence on N. benthamiana plants silenced for NbAGOs 2 and X. Similar results were also obtained upon silencing of NbAGO2 using hairpin vector techniques. Comparable observations were also made when Tobacco mosaic virus GFP constructs were agroinfiltrated on NbAGO2 silenced plants further hinting the antiviral defense roles played by these AGOs. Agroinfiltration of Foxtailmosaic virus, Sunnhemp mosaic virus, and Turnip crinkle virus GFP chimeric constructs on NbAGO2 silenced N. benthamiana plants, however did not result in accumulation of GFP indicating the AGO antiviral defense specificity to TBSV and TMV. The results also hinted at a role for AGO7. Collectively my findings suggest that the expression of AGOs in N. benthamiana is tissue and age dependent, and that unlike in the model plant Arabidopsis where the main antiviral AGO is thought to be AtAGO1; in N. benthamiana, NbAGOs 2 and X seem to be i…
  note: by Denis Odokonyero.
  note: "Major Subject: Plant Pathology"
  note: Includes vita.
  note: Master of Science Texas A & M University 2012 http://hdl.handle.net/1969.1/148228
  note: Includes bibliographical references.
  note: Text (Thesis)
  subject:
    topic: Major Plant Pathology
  subject:
    topic: hairpin vectors
  subject:
    topic: virus induced gene silencing
  subject:
    topic: antiviral RNA silencing
  subject:
    topic: post transcriptional gene silencing
  subject:
    topic: ARGONAUTE
  subject:
    topic: Nicotiana benthamiana
  subject:
    topic: RNAi
  identifier: http://hdl.handle.net/1969.1/148228
  record info:
    record content source: TXA
    record creation date: 131211
    record change date: 20151206090610.0
    record identifier: in00003420396
    language of cataloging:
      language term: eng