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Affinity probe capillary electrophoresis evaluation of aptamer binding to Campylobacter jejuni bacteria /

In this work, we developed a capillary electrophoresis immunoassay to evaluate aptamer binding to bacterial cells. This work is the first known demonstration of aptamer affinity probe capillary electrophoresis to a whole cell target. The aptamer probes investigated were developed against a Campyloba...

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Bibliographic Details
Main Author: Stratis-Cullum, Dimitra N.
Corporate Author: U.S. Army Research Laboratory
Other Authors: McMasters, Sun, Pellegrino, Paul M.
Format: Government Document eBook
Language:English
Published: Adelphi, MD : Army Research Laboratory, [2009]
Series:ARL-TR (Aberdeen Proving Ground, Md.) ; 5015.
Subjects:
Medicine and Medical Research.
Microbiology.
Pathogenic microorganisms.
Jejunum.
Escherichia coli.
Immunoassay.
Food.
Bioassay.
Electrophoresis.
Salmonella.
Shifting.
Capillarity.
Bacteria.
Campylobacter jejuni.
APTAMER
CAMPYLOBACTER JEJUNI
AFFINITY PROBES
CAPILLARY ELECTROPHORESIS
FOOD PATHOGEN
Online Access:http://purl.fdlp.gov/GPO/gpo31787
http://handle.dtic.mil/100.2/ADA512469
Description
Summary:In this work, we developed a capillary electrophoresis immunoassay to evaluate aptamer binding to bacterial cells. This work is the first known demonstration of aptamer affinity probe capillary electrophoresis to a whole cell target. The aptamer probes investigated were developed against a Campylobacter jejuni bacterial cell target, and we evaluated binding relative to other common food-borne pathogens. Specific binding affinity was evidenced by a pronounced mobility shift and peak broadening with increasing bacteria concentration. Little to no mobility shift was observed for food-borne pathogens, Salmonella typhirium and Escherichia coli, even when increasing concentrations 10-fold over target. These results suggest that affinity probe capillary electrophoresis could be useful for qualitative screening of aptamer candidates to bacterial cell targets.
Item Description:Title from title screen (viewed on Nov. 11, 2012).
"November 2009."
Electronic resource.
Physical Description:1 online resource (vi, 14 pages) : illustrations (some color).
Bibliography:Includes bibliographical references (pages 9-12).
Access:APPROVED FOR PUBLIC RELEASE.