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121128s2012 txu obm 000 0 eng d |
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|a (OCoLC)ocn819545975
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|a (OCoLC)819545975
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|a (TxCM)http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11859
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|a TXA
|c TXA
|d UtOrBLW
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|a TXAM
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|a 2012
|a Dissertation
|a 1969.1/ETD-TAMU-2012-08-11859
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| 100 |
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|a Jones, R. Alan
|q (Richard Alan)
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| 245 |
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|a Regulation of thyrotropin mRNA expression in red drum, Sciaenops ocellatus /
|c by Richard Alan Jones.
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|a [College Station, Tex.] :
|b [Texas A&M University],
|c [2012]
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|a 1 online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 500 |
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|a "Major Subject: Zoology"
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|a Description from author supplied metadata (automated record created 2012-10-22 13:24:58).
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| 502 |
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|b Doctor of Philosophy
|c Texas A&M University
|d 2012
|o http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11859
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|a Includes bibliographical references.
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|a Text (Dissertation)
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|a The role of thyroid-stimulating hormone (TSH) in the regulation of peripheral thyroid function in non-mammalian species is still poorly understood. Thyroxine (T₄), the principal hormone released from the thyroid gland in response to TSH stimulation, circulates with a robust daily rhythm in the sciaenid fish, red drum. Previous research has suggested that the red drum T₄ cycle is circadian in nature, driven by TSH secretion in the early photophase and inhibited by T₄ feedback in the early scotophase. To determine whether TSH is produced in a pattern consistent with driving this T₄ cycle, I developed quantitative real time RT-PCR (qPCR) techniques to quantify the daily cycle of expression of the pituitary TSH subunits GSU[alpha], and TSH[beta]. I found that pituitary TSH expression cycled inversely to, and 6-12 hours out of phase with, the T₄ cycle, consistent with the hypothesis that TSH secretion drives the T₄ cycle. To examine the potential role of deiodinases in negative feedback regulation of this TSH cycle, I also utilized qPCR to assess the pituitary expression patterns of the TH activating enzyme outer-ring deiodinase (Dio2) and the TH deactivating enzyme inner ring deiodinase (Dio3). Whereas Dio2 was not expressed with an obvious daily cycle, Dio3 was expressed in the pituitary mirroring the TSH cycle. These results are consistent with T₄ negative feedback on TSH and suggest that TH inactivation by pituitary cells is an important component of the negative feedback system. To further examine the TH regulation of this Dio3 cycle, I developed an immersion technique to administer physiological doses of T₃ and T₄ in vivo. Both hormones persist in static tank water for at least 40 hours. Immersion in 200ng/ml T₄ significantly increased both plasma T₄ and T₃ within physiological ranges above control at 4.5 hours. Immersion in 100ng/ml T₃ increased plasma T₃ within physiological ranges over control by 22 hours while significantly decreasing plasma T₄ below control, presumably through inhibition of TSH secretion. T₄ also significantly inhibited the expression of the TSH [alpha] and [beta] subunits at 4.5 and 22 hours of immersion whereas T₃ immersion significantly inhibited the expression of the [alpha] and [beta] subunits of TSH by 22 hours. Both Dio2 and Dio3 expression were significantly diminished by T3 and T₄ at 22 hours. Inhibition of circulating THs with the goitrogen methimazole significantly increased the expression of TSH. These results indicate that both T₄ and T₃ are capable of negative feedback regulation of TSH expression in red drum on a time scale consistent with the T₄ daily cycle, and further support Dio3 destruction of THs in the pituitary, potentially regulated by circulating T₄, as a critical component of negative feedback on TSH. This study supports the importance of central mechanisms acting through pituitary TSH secretion in regulating thyroid function in red drum.
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|a Electronic resource.
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| 650 |
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|a Major Zoology.
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|a Thyrotropin
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|a Thyroid hormone
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| 653 |
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|a mRNA expression
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| 653 |
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|a Deiodinase
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| 653 |
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|a TSH
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| 653 |
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|a Red drum
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| 700 |
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|a MacKenzie, Duncan S.,
|e thesis advisor.
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| 856 |
4 |
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|u http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11859
|z Link to OAK Trust copy
|t 0
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| 994 |
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|a C0
|b TXA
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| 948 |
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|a cataloged
|b h
|c 2012/11/28
|d o
|e jlanham
|f 10:16:23 am
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| 999 |
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|a MARS
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| 999 |
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|t 0
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| 952 |
f |
f |
|a Texas A&M University
|b College Station
|c Electronic Resources
|d Available Online
|t 0
|e 2012 Dissertation 1969.1/ETD-TAMU-2012-08-11859
|h Other scheme
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| 998 |
f |
f |
|a 2012 Dissertation 1969.1/ETD-TAMU-2012-08-11859
|t 0
|l Available Online
|