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121127s2012 txu obm 000 0 eng d |
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|a (OCoLC)ocn819413520
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|a (OCoLC)819413520
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|a (TxCM)http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9871
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|a TXA
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|a TXAM
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|a 2011
|a Dissertation
|a 1969.1/ETD-TAMU-2011-08-9871
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|a Lee, Anthony Chien-der.
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|a Development of the ultrashort pulse Nonlinear Optical Microscopy Spectral Imaging System /
|c by Anthony Chien-der Lee.
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|a [College Station, Tex.] :
|b [Texas A&M University],
|c [2012]
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|a 1 online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a "Major Subject: Biomedical Engineering"
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|a Description from author supplied metadata (automated record created 2012-10-22 13:24:58).
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|b Doctor of Philosophy
|c Texas A&M University
|d 2011
|o http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9871
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|a Includes bibliographical references.
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|a Text (Dissertation)
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|a Nonlinear Optical Microscopy (NLOM) has been shown to be a valuable tool for noninvasive imaging of complex biological systems. An effective approach for multicolor molecular microscopy is simultaneous excitation of multiple fluorophores by broadband sub-10-fs pulses. This dissertation will discuss the development of two spectral imaging systems using the principles of nonlinear optical microscopy for pixel-by-pixel spectral segmentation of multiple fluorescent spectra. The first spectral system is reliant on a fiber-optic cable to transmit fluorescent signal to a spectrometer, while the second is based on a spectrometer with an aberration-corrected concave grating that is directly coupled to the microscope. A photon-counting, 16-channel multianode photomultiplier tube (PMT) is used for both systems. Custom software developed in LabVIEW controls multiple counter cards as well as a field-programmable gate array (FPGA) for 1 Hz acquisition of 256x256x16 spectral images. Biological specimens consisting of multicolor endothelial cells and zebrafish will be used for experimental verification. Results indicate successful spectral segmentation of multiple fluorophores with a decrease in signal-to-noise ratio in the FPGA-based imaging system.
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|a Electronic resource.
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|a Major Biomedical Engineering.
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|a second harmonic
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|a microscopy
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|a spectral
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|a optical
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|a imaging
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|a nonlinear
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|a multiphoton
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|a NOMSIS
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|a two photon
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|a Yeh, Alvin T.,
|e thesis advisor.
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|u http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9871
|z Link to OAK Trust copy
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|c 2012/11/27
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|a Texas A&M University
|b College Station
|c Electronic Resources
|d Available Online
|t 0
|e 2011 Dissertation 1969.1/ETD-TAMU-2011-08-9871
|h Other scheme
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|a 2011 Dissertation 1969.1/ETD-TAMU-2011-08-9871
|t 0
|l Available Online
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