rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Delivery of proteins in live cells with viral peptides
    sub title: principles and mechanisms
  name:
    name part: Lee, Ya-Jung.
    role:
      role term: creator
  name:
    name part: Pellois, Jean-Philippe
    role:
      role term: thesis advisor.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2012
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: Cell-penetrating peptides (CPPs) mediate the delivery of macromolecules across the plasma membrane of live cells. These peptides are therefore important due to the potential of making the delivery of protein probes or therapeutics a routine procedure. However, CPP-mediated delivery is currently an inefficient process. CPP-protein conjugates are internalized into cells by endocytosis and the macromolecules remain trapped inside endosomes instead of reaching the target cellular localization. To solve this problem, we report a delivery methodology which relies on the use of a chimera of the TAT and of the Influenza HA2. TAT is a prototypical CPP that can promote macropinocytosis in live cells and HA2 is a pH-sensitive peptide that destabilizes lipid membranes upon acidification. I demonstrate that HA2-TAT can deliver a variety of macromolecular cargos into live mammalian cells by a simple co-incubation protocol. A model is described where TAT causes the endocytic uptake of cargos present in the media and that HA2 disrupts the endosomal membrane upon endosomal acidification. In addition, using red blood cells as a model system, HA2-TAT binds to membranes in a pH-dependent manner and causes the formation of pores through which macromolecules can diffuse. Additionally, the pro-apoptotic domain (PAD) peptide is also successfully delivered by HA2-TAT and shows significant apoptosis in cells through macropinocytosis.
  note: by Ya-Jung Lee.
  note: "Major Subject: Biochemistry"
  note: Electronic resource.
  note: Doctor of Philosophy Texas A&M University 2011 http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9270
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Biochemistry
  subject:
    topic: hemolysis
  subject:
    topic: TAT
  subject:
    topic: mCherry
  subject:
    topic: HA2
  identifier: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9270
  record info:
    record content source: TXA
    record creation date: 120808
    record change date: 20150922105907.0
    record identifier: in00002779743