rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Molecular typing and antimicrobial resistance of Campylobacter isolated during commercial broiler production
  name:
    name part: Hernandez, Charles Andrew.
    role:
      role term: creator
  name:
    name part: Caldwell, David J.
    role:
      role term: thesis advisor.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2012
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: Campylobacter jejuni is a commensal microorganism of the poultry gastrointestinal tract. Broilers, layers, ducks, turkeys, and quails can be colonized by Campylobacter without illness occurring. The vast majority of human Campylobacter infections are recognized as being foodborne. For 2008, preliminary FoodNet data showed that the Campylobacter incidence of infection,12.68 per 100,000 of the U.S. population, is the second highest, only behind Salmonella at 16.20 per 100,000. To further understand Campylobacter's role as a foodborne pathogen, analysis at the molecular level is needed. Microbial molecular typing allows for identification and differentiation of bacterial strains beneath the species level. In this study, the "gold standard" method for molecular subtyping, Pulsed Field Gel Electrophoresis (PFGE), along with Diversilab® repetitive element Polymerase Chain Reaction (rep-PCR) and 16S-23S Internal Spacer Region Denaturing Gradient Gel Electrophoresis (ISR DGGE) were used for the molecular typing of Campylobacter jejuni isolates obtained during different stages of commercial broiler production and processing. In addition, the C. jejuni isolates were tested for resistance to antimicrobials commonly used in both veterinary and human medicine. Antimicrobial resistance testing was carried out using a broth dilution system. The majority of recovered isolates came from post-harvest carcass rinsates. Carcass rinses were obtained at post-evisceration, post-chill stages. All isolates (n = 46) were identified by the Polymerase Chain Reaction as Campylobacter jejuni. Three genotypes (n = 44, n = 1, n = 1) were identified by PFGE. The 46 rep-PCR products grouped into seven clusters and two outliers. Clustering of rep-PCR products by sample source was not observed. No relatedness trends were observed for isolates recovered from the same source. The combination of PFGE and Diversilab rep-PCR methods provides highly discriminatory molecular typing results. These results pro…
  note: by Charles Andrew Hernandez, Jr.
  note: "Major Subject: Poultry Science"
  note: Electronic resource.
  note: Master of Science Texas A&M University 2010 http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8643
  note: Includes bibliographical references.
  note: Text (Thesis)
  subject:
    topic: Major poultry science
  subject:
    topic: PFGE
  subject:
    topic: antimicrobial resistance
  subject:
    topic: Campylobacter
  subject:
    topic: rep-PCR
  identifier: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8643
  record info:
    record content source: TXA
    record creation date: 120322
    record change date: 20151202151912.0
    record identifier: in00002743764