rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Simulation of contamination through the post-harvest environment using surrogate organisms
  name:
    name part: Villarreal Silva, Mariana.
    role:
      role term: creator
  name:
    name part: Hardin, Margaret D.
    role:
      role term: thesis advisor.
  name:
    name part: Castillo, Alejandro
    role:
      role term: thesis advisor.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2011
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: The beef industry has made tremendous strides in reducing pathogen contamination on carcasses. Multiple antimicrobial interventions have been validated for their use during harvesting. Information in regards to cross-contamination with pathogens in the post-harvest environment is limited. Surrogate microorganisms for enteric pathogens are commonly used to validate antimicrobial interventions and might allow for the simulation of cross-contamination through the post-harvest environment. The purpose of this study was to determine how the post-harvest environment impacts the direct and indirect transmission of pathogens. This was achieved by using fluorescent protein-marked surrogate strains of Escherichia coli O157:H7 and Salmonella spp. from inoculated carcasses to the adjacent ones and to the equipment and facility in three different abattoirs. Thirteen hide-on carcasses were inoculated using a gelatin-based slurry containing three nonpathogenic fluorescent protein-marked strains of E. coli biotype I. In order to determine direct and indirect cross-contamination, inoculated and adjacent carcasses were sampled (300 cm²) during the harvesting process at different stages: after hide opening (AHO), prior to evisceration (PE), after evisceration (AE), after splitting (AS), and after final intervention (AFI). Environmental samples consisting of the floor, walls, and air were tested as well as personal equipment including gloves, boots, and aprons. Equipment including hand knives, air knives, meat hooks, hide puller and split saw were also sampled. Results showed evidence of cross-contamination between inoculated carcasses and the adjacent non-inoculated ones for all abattoirs. Although this occurred in all abattoirs, surrogate counts on carcasses were below detectable levels (<1.4 log CFU/cm²) after antimicrobial interventions. Surrogates were found in low levels for all environmental samples. However surrogate counts from equipment such as knives, split saws, meat hooks,…
  note: by Mariana Villarreal Silva.
  note: "Major Subject: Food Science and Technology"
  note: Title from author supplied metadata (automated record created 2011-11-01 09:04:20).
  note: Electronic resource.
  note: Master of Science Texas A&M University 2010 http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8542
  note: Includes bibliographical references.
  note: Text (Thesis)
  subject:
    topic: Major food science and technology
  subject:
    topic: Escherichia coli O157:H7
  subject:
    topic: pathogen
  subject:
    topic: Salmonella
  subject:
    topic: beef
  subject:
    topic: contamination
  subject:
    topic: surrogate
  identifier: http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8542
  record info:
    record content source: TXA
    record creation date: 111116
    record change date: 20151202144306.0
    record identifier: in00002721240